• Alteration in mitochondrial Ca2+ uptake disrupts insulin signaling in hypertrophic cardiomyocytes 

      Gutiérrez, Tomás; Parra, Valentina; Troncoso, Rodrigo; Pennanen, Christian; Contreras Ferrat, Ariel Eduardo; Vásquez Trincado, César Alonso; Morales, Pablo E.; López Crisosto, Camila; Sotomayor Flores, Cristian Alejandro; Chiong Lay, Mario; Rothermel, Beverly A.; Lavandero González, Sergio (BioMed Central Ltd., 2014)
      © 2014 Gutierrez et al.; licensee BioMed Central Ltd. Background: Cardiac hypertrophy is characterized by alterations in both cardiac bioenergetics and insulin sensitivity. Insulin promotes glucose uptake by cardiomyocytes ...
    • Anabolic Androgenic Steroids and Intracellular Calcium Signaling: A Mini Review on Mechanisms and Physiological Implications 

      Vicencio Bustamante, José Manuel; Estrada, M.; Galvis, D.; Bravo, R.; Contreras Ferrat, Ariel Eduardo; Rotter, D.; Szabadkai, G.; Hill, Joseph A.; Rothermel, B. A.; Jaimovich Pérez, Enrique; Lavandero González, Sergio (BENTHAM SCIENCE PUBL LTD, 2011-05)
      Increasing evidence suggests that nongenomic effects of testosterone and anabolic androgenic steroids (AAS) operate concertedly with genomic effects. Classically, these responses have been viewed as separate and independent ...
    • Autofagia del cardiomiocito: ¿Un nuevo mecanismo de adaptación al estrés o de muerte celular? 

      Aránguiz Urroz, Pablo Daniel; Contreras Ferrat, Ariel Eduardo; Rojas Rivera, Diego; Troncoso Cotal, Rodrigo Hernán; Marambio Morales, Paola Angélica; Toro, Bárbara; Sanhueza Muñoz, Carlos Joaquín; Pennanen Saavedra, Christian; Díaz Elizondo, Jessica; Parra Ortíz, María Valentina; Chiong Lay, Mario; Díaz Araya, Guillermo; Lavandero González, Sergio (Sociedad Chilena de Cardiología y Cirugía Cardiovascular, 2006)
      Históricamente, la apoptosis y la necrosis han sido consideradas como las dos formas fundamentales de muerte celular. Sin embargo, evidencias recientes sugieren que la muerte celular programada no está confinada sólo a ...
    • Ca2+ signals promote GLUT4 exocytosis and reduce its endocytosis in muscle cells 

      Li, Q.; Zhu, X.; Ishikura, S.; Zhang, D.; Gao, J.; Sun, Y.; Contreras Ferrat, Ariel Eduardo; Foley, K. P.; Lavandero González, Sergio; Yao, Z.; Bilan, P. J.; Klip, A.; Niu, W. (American Physiological Society, 2014)
      Ca2+ signals promote GLUT4 exocytosis and reduce its endocytosis in muscle cells. Am J Physiol Endocrinol Metab 307: E209–E224, 2014. First published June 3, 2014; doi:10.1152/ajpendo.00045.2014.— Elevating cytosolic ...
    • Cav1.1 controls frequency-dependent events regulating adult skeletal muscle plasticity 

      Jorquera, Gonzalo; Altamirano, Francisco; Contreras Ferrat, Ariel Eduardo; Almarza, Gonzalo; Buvinic Radic, Sonja; Jacquemond, Vincent; Jaimovich Pérez, Enrique; Casas Atala, Mariana (The Company of Biologists, 2013)
      An important pending question in neuromuscular biology is how skeletal muscle cells decipher the stimulation pattern coming from motoneurons to define their phenotype as slow or fast twitch muscle fibers. We have previously ...
    • Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle 

      Arias Calderón, Manuel; Almarza, Gonzalo; Díaz Vegas, Alexis; Contreras Ferrat, Ariel Eduardo; Valladares Ide, Denisse; Casas Atala, Mariana; Toledo Araya, Héctor; Jaimovich Pérez, Enrique; Buvinic Radic, Sonja (Biomed Central, 2016)
      Background: Electrical activity regulates the expression of skeletal muscle genes by a process known as “ excitation-transcription” (E-T) coupling. We have demonstrated that release of adenosine 5′-triphosphate (ATP) ...
    • Contraction-related stimuli regulate GLUT4 traffic in C2C 12-GLUT4myc skeletal muscle cells 

      Niu, Wenyan; Bilan, Philip J.; Ishikura, Shuhei; Schertzer, Jonathan D.; Contreras-Ferrat, Ariel; Fu, Zhengxiang; Liu, Jie; Boguslavsky, Shlomit; Foley, Kevin P.; Liu, Zhi; Li, Jinru; Chu, Guilan; Panakkezhum, Thomas; Lopaschuk, Gary D.; Lavandero González, Sergio; Yao, Zhi; Klip (2010)
      Muscle contraction stimulates glucose uptake acutely to increase energy supply, but suitable cellular models that faithfully reproduce this complex phenomenon are lacking. To this end, we have developed a cellular model ...
    • Contraction-related stimuli regulate GLUT4 traffic in C2C12-GLUT4myc skeletal muscle cells 

      Niu, Wenyan; Bilan, Philip J.; Ishikura, Shuhei; Schertzer, Jonathan D.; Contreras Ferrat, Ariel Eduardo; Fu, Zhengxiang; Liu, Jie; Boguslavsky, Shlomit; Foley, Kevin P.; Liu, Zhi; Li, Jinru; Chu, Guilan; Panakkezhum, Thomas; Lopaschuk, Gary D.; Lavandero González, Sergio; Yao, Zhi; Klip, Amira (2010)
      Contraction-related stimuli regulate GLUT4 traffic in C2C12- GLUT4myc skeletal muscle cells. Am J Physiol Endocrinol Metab 298: E1058 –E1071, 2010. First published February 16, 2010; doi:10.1152/ajpendo.00773.2009.—Muscle ...
    • Differential Participation of Angiotensin II Type 1 and 2 Receptors in the Regulation of Cardiac Cell Death Triggered by Angiotensin II 

      Aránguiz Urroz, Pablo Daniel; Soto Muñoz, Dagoberto; Contreras Ferrat, Ariel Eduardo; Troncoso Cotal, Rodrigo Andrés; Chiong Lay, Mario; Montenegro Obregón, José Joaquín; Venegas Pino, Daniel Esteban; Smolic Smolic, Christian; Ayala Jeria, Pedro Segundo; Thomas, W. G.; Lavandero González, Sergio; Díaz Araya, Guillermo (NATURE PUBLISHING GROUP, 2009-05)
      The Angiotensin II (Ang II) type 1 (AT(1)R) and type 2 (AT(2)R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation ...
    • Electrical Stimuli Release ATP to Increase GLUT4 Translocation and Glucose Uptake via PI3Kg-Akt-AS160 in Skeletal Muscle Cells 

      Osorio Fuentealba, César; Contreras Ferrat, Ariel Eduardo; Altamirano, Francisco; Espinosa Escalona, Berta; Li, Qing; Niu, Wenyan; Lavandero González, Sergio; Klip, Amira; Jaimovich Pérez, Enrique (American Diabetes Association, 2013)
      Skeletal muscle glucose uptake in response to exercise is preserved in insulin-resistant conditions, but the signals involved are debated. ATP is released from skeletal muscle by contractile activity and can autocrinely ...
    • Glucose-Dependent Insulin Secretion in Pancreatic beta-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors 

      Llanos Vidal, Paola; Contreras Ferrat, Ariel Eduardo; Barrientos Briones, Genaro; Valencia, Marco; Mears, David; Hidalgo Tapia, María Cecilia (Public Library Science, 2015)
      Glucose-stimulated insulin secretion (GSIS) from pancreatic beta-cells requires an increase in intracellular free Ca2+ concentration ([Ca2+]). Glucose uptake into beta-cells promotes Ca2+ influx and reactive oxygen species ...
    • IGF-1: Un factor de crecimiento con acciones cardiovasculares pleiotrópicas. 

      Contreras Ferrat, Ariel Eduardo; Aránguiz Urroz, Pablo Daniel; Díaz Elizondo, Jessica; Chiong Lay, Mario; Muñoz, Juan Pablo; Parra Ortíz, María Valentina; Ibarra, Cristián; Rojas Rivera, Diego; Troncoso Cotal, Rodrigo; Eisner, Verónica; Salas, Daniela; Rodríguez Villarroel, Andrea Elizabeth; Carrasco Alarcón, Loreto del Pilar; Díaz Araya, Guillermo; Lavandero González, Sergio (Sociedad Chilena de Cardiología y Cirugía Cardiovascular, 2006)
      El factor de crecimiento análogo a insulina tipo 1 (IGF-1) es un péptido relacionado estructural y funcionalmente con insulina que posee efectos mitogénicos y citoprotectores. Sus efectos biológicos dependen de la activación ...
    • An Inositol 1,4,5-Triphosphate (IP3)-IP3 Receptor Pathway Is Required for Insulin-Stimulated Glucose Transporter 4 Translocation and Glucose Uptake in Cardiomyocytes 

      Contreras Ferrat, Ariel Eduardo; Toro, Barbra; Bravo, R.; Parra Ortíz, María Valentina; Vásquez, C.; Ibarra, Cristián; Mears, David; Chiong Lay, Mario; Jaimovich Pérez, Enrique; Klip, Amira; Lavandero González, Sergio (ENDOCRINE SOC, 2010-10)
      Intracellular calcium levels ([Ca2+](i)) and glucose uptake are central to cardiomyocyte physiology, yet connections between them have not been studied. We investigated whether insulin regulates [Ca2+](i) in cultured ...
    • Insulin elicits a ROS-activated and an IP3-dependent Ca2+ release, which both impinge on GLUT4 translocation 

      Contreras Ferrat, Ariel Eduardo; Llanos Vidal, Paola; Vásquez, César; Espinosa, Alejandra; Osorio Fuentealba, César; Arias Calderón, Manuel; Lavandero González, Sergio; Klip, Amira; Hidalgo Tapia, María Cecilia; Jaimovich Pérez, Enrique (The Company of Biologists Ltd, 2014)
      Insulin signaling includes generation of low levels of H2O2; however, its origin and contribution to insulin-stimulated glucose transport are unknown. We tested the impact of H2O2 on insulin-dependent glucose transport ...
    • Insulin-Dependent H2O2 Production Is Higher in Muscle Fibers of Mice Fed with a High-Fat Diet 

      Espinosa, Alejandra; Campos, Cristian; Díaz Vegas, Alexis; Galgani Fuentes, José Eduardo; Juretic , Nevenka; Osorio Fuentealba, César; Bucarey, José L.; Tapia, Gladys; Valenzuela, Rodrigo; Contreras Ferrat, Ariel Eduardo; Llanos, Paola; Jaimovich Pérez, Enrique (2013)
      Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with a high-fat diet (HFD) are a model of insulin resistance. In skeletal muscle, hydrogen peroxide (H2O2) ...
    • Insulina regula los niveles intracelulares de calcio en el cardiomiocito 

      Contreras Ferrat, Ariel Eduardo (Universidad de Chile, 2007)
      Insulina y su receptor (RI) regulan el metabolismo de ácidos grasos y carbohidratos en el corazón. La activación de este receptor por su ligando endógeno desencadena una serie de eventos reguladores de la homeostasis energética ...
    • Mecanismos transduccionales de insulina en el cardiomiocito : papel del calcio en la incorporación de glucosa 

      Contreras Ferrat, Ariel Eduardo (Universidad de ChileCyberDocs, 2010)
      Tanto los niveles intracelulares de Ca2+ ([Ca2+]i) como el transporte de la glucosa son fundamentales para la fisiología del cardiomiocito, sin embargo, aún no se ha investigado la interrelación entre ambos eventos. En ...
    • Mitochondrial calcium increase induced by RyR1 and IP3R channel activation after membrane depolarization regulates skeletal muscle metabolism 

      Díaz Vegas, Alexis; Córdova, A.; Valladares, Denisse; Llanos, Paola; Hidalgo, C.; Gherardi, Gaia; Stefani, Diego De; Mammucari, Cristina; Rizzuto, Rosario; Contreras Ferrat, Ariel Eduardo; Jaimovich Pérez, Enrique (Frontiers Media, 2018-06)
      Aim: We hypothesize that both type-1 ryanodine receptor (RyR1) and IP3-receptor (IP3R) calcium channels are necessary for the mitochondrial Ca2+ increase caused by membrane depolarization induced by potassium (or by ...
    • Mitochondrial fragmentation impairs insulin-dependent glucose uptake by modulating Akt activity through mitochondrial Ca2+ uptake 

      Del Campo Sfeir, Andrea Estefanía; Parra Ortíz, María Valentina; Vásquez Trincado, César Alonso; Gutiérrez, Tomás; Morales, Pablo E.; López Crisosto, Camila; Bravo Sagua, Roberto; Navarro Márquez, Mario F.; Verdejo, Hugo E.; Contreras Ferrat, Ariel Eduardo; Troncoso Cotal, Rodrigo; Chiong Lay, Mario; Lavandero González, Sergio (American Physiological Society, 2014)
      Insulin is a major regulator of glucose metabolism, stimulating its mitochondrial oxidation in skeletal muscle cells. Mitochondria are dynamic organelles that can undergo structural remodeling in order to cope with these ...
    • NOX2 Inhibition Impairs Early Muscle Gene Expression Induced by a Single Exercise Bout 

      Henríquez Olguín, Carlos; Díaz Vegas, Alexis; Utreras Mendoza, Yildy; Campos Acevedo, Cristian; Arias Calderón, Manuel; Llanos Vidal, Paola; Contreras Ferrat, Ariel Eduardo; Espinosa Escalona, Alejandra; Altamirano, Francisco; Jaimovich Pérez, Enrique; Valladares Ide, Denisse (Frontiers Media, 2016)
      Reactive oxygen species (ROS) participate as signaling molecules in response to exercise in skeletal muscle. However, the source of ROS and the molecular mechanisms involved in these phenomena are still not completely ...