• Anabolic Androgenic Steroids and Intracellular Calcium Signaling: A Mini Review on Mechanisms and Physiological Implications 

      Vicencio Bustamante, José Manuel; Estrada, M.; Galvis, D.; Bravo, R.; Contreras Ferrat, Ariel Eduardo; Rotter, D.; Szabadkai, G.; Hill, Joseph A.; Rothermel, B. A.; Jaimovich Pérez, Enrique; Lavandero González, Sergio (BENTHAM SCIENCE PUBL LTD, 2011-05)
      Increasing evidence suggests that nongenomic effects of testosterone and anabolic androgenic steroids (AAS) operate concertedly with genomic effects. Classically, these responses have been viewed as separate and independent ...
    • Autofagia del cardiomiocito: ¿Un nuevo mecanismo de adaptación al estrés o de muerte celular? 

      Aránguiz Urroz, Pablo Daniel; Contreras Ferrat, Ariel Eduardo; Rojas Rivera, Diego; Troncoso Cotal, Rodrigo Hernán; Pennanen Saavedra, Christian; Marambio Morales, Paola Angélica; Sanhueza Muñoz, Carlos Joaquín; Toro, Barbra; Díaz Elizondo, Jessica Alejandra; Parra Ortíz, María Valentina; Díaz Araya, Guillermo; Lavandero González, Sergio; Chiong Lay, Mario (Sociedad Chilena de Cardiología y Cirugía Cardiovascular, 2006)
      Históricamente, la apoptosis y la necrosis han sido consideradas como las dos formas fundamentales de muerte celular. Sin embargo, evidencias recientes sugieren que la muerte celular programada no está confinada sólo a ...
    • Cav1.1 controls frequency-dependent events regulating adult skeletal muscle plasticity 

      Jorquera, Gonzalo; Altamirano, Francisco; Contreras Ferrat, Ariel; Almarza, Gonzalo; Casas Atala, Mariana; Jacquemond, Vincent; Buvinic Radic, Sonja; Jaimovich Pérez, Enrique (The Company of Biologists, 2013)
      An important pending question in neuromuscular biology is how skeletal muscle cells decipher the stimulation pattern coming from motoneurons to define their phenotype as slow or fast twitch muscle fibers. We have previously ...
    • Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle 

      Arias Calderón, Manuel; Almarza, Gonzalo; Díaz Vegas, Alexis; Contreras Ferrat, Ariel; Valladares Ide, Denisse; Casas Atala, Mariana; Toledo Araya, Héctor; Jaimovich Pérez, Enrique; Buvinic Radic, Sonja (Biomed Central, 2016)
      Background: Electrical activity regulates the expression of skeletal muscle genes by a process known as “ excitation-transcription” (E-T) coupling. We have demonstrated that release of adenosine 5′-triphosphate (ATP) ...
    • Contraction-related stimuli regulate GLUT4 traffic in C2C12-GLUT4myc skeletal muscle cells 

      Niu, Wenyan; Bilan, Philip J.; Ishikura, Shuhei; Schertzer, Jonathan D.; Contreras Ferrat, Ariel Eduardo; Fu, Zhengxiang; Liu, Jie; Boguslavsky, Shlomit; Foley, Kevin P.; Liu, Zhi; Li, Jinru; Chu, Guilan; Panakkezhum, Thomas; Lopaschuk, Gary D.; Lavandero González, Sergio; Yao, Zhi; Klip, Amira (2010)
      Contraction-related stimuli regulate GLUT4 traffic in C2C12- GLUT4myc skeletal muscle cells. Am J Physiol Endocrinol Metab 298: E1058 –E1071, 2010. First published February 16, 2010; doi:10.1152/ajpendo.00773.2009.—Muscle ...
    • Differential Participation of Angiotensin II Type 1 and 2 Receptors in the Regulation of Cardiac Cell Death Triggered by Angiotensin II 

      Aránguiz Urroz, Pablo Daniel; Soto Muñoz, Dagoberto; Contreras Ferrat, Ariel Eduardo; Troncoso Cotal, Rodrigo Andrés; Chiong Lay, Mario; Montenegro Obregón, José Joaquín; Venegas Pino, Daniel Esteban; Smolic Smolic, Christian; Ayala Jeria, Pedro Segundo; Thomas, W. G.; Lavandero González, Sergio; Díaz Araya, Guillermo (NATURE PUBLISHING GROUP, 2009-05)
      The Angiotensin II (Ang II) type 1 (AT(1)R) and type 2 (AT(2)R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation ...
    • Glucose-Dependent Insulin Secretion in Pancreatic beta-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors 

      Llanos Vidal, Paola; Contreras Ferrat, Ariel; Barrientos Briones, Genaro; Valencia, Marco; Mears, David; Hidalgo Tapia, María Cecilia (Public Library Science, 2015)
      Glucose-stimulated insulin secretion (GSIS) from pancreatic beta-cells requires an increase in intracellular free Ca2+ concentration ([Ca2+]). Glucose uptake into beta-cells promotes Ca2+ influx and reactive oxygen species ...
    • IGF-1: Un factor de crecimiento con acciones cardiovasculares pleiotrópicas. 

      Contreras Ferrat, Ariel Eduardo; Aránguiz Urroz, Pablo Daniel; Díaz Elizondo, Jessica Alejandra; Chiong Lay, Mario; Parra Ortíz, María Valentina; Rojas Rivera, Diego; Troncoso Cotal, Rodrigo Hernán; Rodríguez Villarroel, Andrea Elizabeth; Carrasco Alarcón, Loreto del Pilar; Díaz Araya, Guillermo; Lavandero González, Sergio (Sociedad Chilena de Cardiología y Cirugía Cardiovascular, 2006)
      El factor de crecimiento análogo a insulina tipo 1 (IGF-1) es un péptido relacionado estructural y funcionalmente con insulina que posee efectos mitogénicos y citoprotectores. Sus efectos biológicos dependen de la activación ...
    • An Inositol 1,4,5-Triphosphate (IP3)-IP3 Receptor Pathway Is Required for Insulin-Stimulated Glucose Transporter 4 Translocation and Glucose Uptake in Cardiomyocytes 

      Contreras Ferrat, Ariel Eduardo; Toro, Barbra; Bravo, R.; Parra Ortíz, María Valentina; Vásquez, C.; Ibarra, Cristián; Mears, David; Chiong Lay, Mario; Jaimovich Pérez, Enrique; Klip, Amira; Lavandero González, Sergio (ENDOCRINE SOC, 2010-10)
      Intracellular calcium levels ([Ca2+](i)) and glucose uptake are central to cardiomyocyte physiology, yet connections between them have not been studied. We investigated whether insulin regulates [Ca2+](i) in cultured ...
    • Insulin elicits a ROS-activated and an IP3-dependent Ca2+ release, which both impinge on GLUT4 translocation 

      Contreras Ferrat, Ariel; Llanos Vidal, Paola; Vásquez, César; Espinosa, Alejandra; Osorio Fuentealba, César; Espinosa Escalona, Berta; Arias Calderón, Manuel; Lavandero González, Sergio; Klip, Amira; Hidalgo Tapia, María Cecilia; Jaimovich Pérez, Enrique (The Company of Biologists Ltd, 2014)
      Insulin signaling includes generation of low levels of H2O2; however, its origin and contribution to insulin-stimulated glucose transport are unknown. We tested the impact of H2O2 on insulin-dependent glucose transport ...
    • Insulina regula los niveles intracelulares de calcio en el cardiomiocito 

      Contreras Ferrat, Ariel Eduardo (Universidad de Chile, 2007)
      Insulina y su receptor (RI) regulan el metabolismo de ácidos grasos y carbohidratos en el corazón. La activación de este receptor por su ligando endógeno desencadena una serie de eventos reguladores de la homeostasis energética ...
    • Mecanismos transduccionales de insulina en el cardiomiocito : papel del calcio en la incorporación de glucosa 

      Contreras Ferrat, Ariel Eduardo (Universidad de ChileCyberDocs, 2010)
      Tanto los niveles intracelulares de Ca2+ ([Ca2+]i) como el transporte de la glucosa son fundamentales para la fisiología del cardiomiocito, sin embargo, aún no se ha investigado la interrelación entre ambos eventos. En ...
    • Mitochondrial calcium increase induced by RyR1 and IP3R channel activation after membrane depolarization regulates skeletal muscle metabolism 

      Díaz Vegas, Alexis; Córdova, A.; Valladares, Denisse; Llanos, Paola; Hidalgo, C.; Gherardi, Gaia; Stefani, Diego De; Mammucari, Cristina; Rizzuto, Rosario; Contreras Ferrat, Ariel; Jaimovich Pérez, Enrique (Frontiers Media, 2018-06)
      Aim: We hypothesize that both type-1 ryanodine receptor (RyR1) and IP3-receptor (IP3R) calcium channels are necessary for the mitochondrial Ca2+ increase caused by membrane depolarization induced by potassium (or by ...
    • Mitochondrial fragmentation impairs insulin-dependent glucose uptake by modulating Akt activity through mitochondrial Ca2+ uptake 

      Del Campo Sfeir, Andrea Estefanía; Parra Ortíz, María Valentina; Vásquez Trincado, César Alonso; Gutiérrez, Tomás; Morales, Pablo E.; López Crisosto, Camila Paz; Bravo Sagua, Roberto Francisco; Navarro Márquez, Mario F.; Verdejo, Hugo E.; Contreras Ferrat, Ariel Eduardo; Troncoso Cotal, Rodrigo Hernán; Chiong Lay, Mario Martín; Lavandero González, Sergio (American Physiological Society, 2014)
      Insulin is a major regulator of glucose metabolism, stimulating its mitochondrial oxidation in skeletal muscle cells. Mitochondria are dynamic organelles that can undergo structural remodeling in order to cope with these ...
    • NOX2 Inhibition Impairs Early Muscle Gene Expression Induced by a Single Exercise Bout 

      Henríquez Olguín, Carlos; Díaz Vegas, Alexis; Utreras Mendoza, Yildy; Campos Acevedo, Cristian; Arias Calderón, Manuel; Llanos Vidal, Paola; Contreras Ferrat, Ariel; Espinosa Escalona, Alejandra; Altamirano, Francisco; Jaimovich Pérez, Enrique; Valladares Ide, Denisse (Frontiers Media, 2016)
      Reactive oxygen species (ROS) participate as signaling molecules in response to exercise in skeletal muscle. However, the source of ROS and the molecular mechanisms involved in these phenomena are still not completely ...
    • NOX2-Dependent ROS Production is Essential for the Response to Endurance Exercise in Skeletal Muscle 

      Henríquez Olguín, Carlos; Valladares Ide, Denisse; Díaz Vegas, Alexis; Utreras Mendoza, Yildy; Campos, Cristian; Llanos, Paola; Contreras Ferrat, Ariel; Espinosa Escalona, Alejandra; Altamirano, Francisco; Arias Calderón, Manuel (Federation of American Societies for Experimental Biology, 2015)
    • Polycystin-1 Is a Cardiomyocyte Mechanosensor That Governs L-Type Ca2+ Channel Protein Stability 

      Pedrozo Cibils, Zully; Criollo Céspedes, Alfredo; Battiprolu, Pavan K.; Morales, Cyndi R.; Contreras Ferrat, Ariel; Fernández, Carolina; Jiang, Nan; Luo, Xiang; Caplan, Michael J.; Somlo, Stefan; Rothermel, Beverly A.; Gillette, Thomas G.; Lavandero González, Sergio; Hill, Joseph A. (Lippicott Williams & Wilkins, 2015)
      Background-L-type calcium channel activity is critical to afterload-induced hypertrophic growth of the heart. However, the mechanisms governing mechanical stress-induced activation of L-type calcium channel activity are ...
    • Potential role of skeletal muscle glucose metabolism on the regulation of insulin secretion 

      Mizgier, M. L.; Casas Atala, Mariana; Contreras Ferrat, Ariel Eduardo; Llanos Vidal, Paola; Galgani, J. E. (2014)
      Pancreatic beta cells sense glucose flux and release as much insulin as required in order to maintain glycaemia within a narrow range. Insulin secretion is regulated by many factors including glucose, incretins, and ...
    • Regulatory volume decrease in cardiomyocytes is modulated by calcium influx and reactive oxygen species 

      Rojas Rivera, Andrés; Díaz Elizondo, Jessica Alejandra; Parra Ortíz, María Valentina; Salas, Daniela; Contreras Ferrat, Ariel Eduardo; Toro, Barbra; Chiong Lay, Mario; Olea Azar, Claudio; Lavandero González, Sergio (ELSEVIER SCIENCE BV, 2009-11-03)
      We investigated the role of Ca2+ in generating reactive oxygen species (ROS) induced by hyposmotic stress (Hypo) and its relationship to regulatory volume decrease (RVD) in cardiomyocytes. Hypo-induced increases in cytoplasmic ...
    • ROS Production via P2Y(1)-PKC-NOX2 Is Triggered by Extracellular ATP after Electrical Stimulation of Skeletal Muscle Cells 

      Díaz Vegas, Alexis; Campos, Cristian A.; Contreras Ferrat, Ariel; Casas Atala, Mariana; Buvinic Radic, Sonja; Jaimovich Pérez, Enrique; Espinosa Escalona, Alejandra (Public Library Science, 2015)
      During exercise, skeletal muscle produces reactive oxygen species (ROS) via NADPH oxidase (NOX2) while inducing cellular adaptations associated with contractile activity. The signals involved in this mechanism are still a ...