Now showing items 1-20 of 36

    • A method for analysis of lipid vesicle domain structure from confocal image data 

      Husen, Peter; Fidorra, Matthias; Härtel, Steffen; Bagatolli, Luis A.; Ipsen, Jhon H. (Springer, 2011-11-09)
      Quantitative characterization of the lateral structure of curved membranes based on fluorescence microscopy requires knowledge of the fluorophore distribution on the surface. We present an image analysis approach for ...
    • Asymmetric morphogenesis of the parapineal organ in the embryonic zebrafish brain 

      Lemus Cortés, Carmen; Palma, Karina; Jara, Jorge; Cramer, Teresa; Härtel, Steffen; Concha Nordemann, Miguel (Elsevier, 2017-07)
    • Aumento del daño en el ADN espermático en varones mayores de 40 años 

      Horta, Fabrizzio; Madariaga, Marcia; García, Alejandra; Härtel, Steffen; Smith Garcés, Rosita (SOC MEDICA SANTIAGO, 2011-03)
      Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two ...
    • Automatic classification of human sperm head morphology 

      Chang, Violeta; Heutte, Laurent; Petitjean, Caroline; Härtel, Steffen; Hitschfeld, Nancy (Elsevier, 2017)
      Background and objective: Infertility is a problem that affects up to 15% of couples worldwide with emotional and physiological implications and semen analysis is the first step in the evaluation of an infertile couple. ...
    • Bleomycin-induced γH2AX foci map preferentially to replicating domains in CHO9 interphase nuclei 

      Liddle, Pablo; Lafon-Hughes, Laura; Di Tomaso, María Vittoria; Reyes-Ábalos, Ana Laura; Jara, Jorge; Cerda, Mauricio; Härtel, Steffen; Folle, Gustavo A. (Kluwer Academic Publishers, 2014)
      © 2014, Springer Science+Business Media Dordrecht.Exposure to DNA damaging agents triggers phosphorylation of histone variant H2AX (generating γH2AX) in large chromatin regions flanking DNA lesions, allowing their ...
    • Caveolin-1-Enhanced Motility and Focal Adhesion Turnover Require Tyrosine-14 but Not Accumulation to the Rear in Metastatic Cancer Cells 

      Urra, Hery; Torres Gómez, Vicente; Ortiz, Rina J.; Lobos, Lorena; Díaz, María I.; Díaz, Natalia; Härtel, Steffen; Leyton Campos, Lisette; Quest, Andrew F. G. (PUBLIC LIBRARY SCIENCE, 2012-04-10)
      Caveolin-1 is known to promote cell migration, and increased caveolin-1 expression is associated with tumor progression and metastasis. In fibroblasts, caveolin-1 polarization and phosphorylation of tyrosine-14 are essential ...
    • CDK5 downregulation enhances synaptic plasticity 

      Posada Duque, Rafael; Ramírez, Omar A.; Härtel, Steffen; Inestrosa, Nibaldo C.; Bodaleo, Felipe; González Billault, Christian; Kirkwood, Alfredo; Cardona Gómez, Gloria (Birkhauser Verlag AG, 2017)
      © 2016, Springer International Publishing. CDK5 is a serine/threonine kinase that is involved in the normal function of the adult brain and plays a role in neurotransmission and synaptic plasticity. However, its over-regulation ...
    • Changes in Rab3D Expression and Distribution in the Acini of Sjogren's Syndrome Patients Are Associated With Loss of Cell Polarity and Secretory Dysfunction 

      Bahamondes, Verónica; Albornoz, Amelina; Aguilera, Sergio; Alliende, Cecilia; Molina, Claudio; Castro, Isabel; Urzúa Tobar, Ulises; Quest, Andrew F. G.; Barrera, María José; González, Sergio; Sánchez, Marianela; Härtel, Steffen; Hermoso Ramello, Marcela; Leyton Martínez, Cecilia; González Burgos, María Julieta (WILEY-BLACKWELL, 2011-10)
      Objective. Oral and ocular dryness are frequent and serious symptoms of Sjogren's syndrome (SS) that reflect problems in secretion due to glandular dysfunction. Exocytosis, an important process in the secretory pathway, ...
    • Changes in Rab3D expression and distribution in the acini of Sjögren's syndrome patients are associated with loss of cell polarity and secretory dysfunction 

      Bahamondes, Verónica; Albornoz, Amelina; Aguilera, Sergio; Alliende, Cecilia; Molina, Claudio; Castro, Isabel; Urzúa, Ulises; Quest, Andrew F. G.; Barrera, María José; González, Sergio; Sánchez, Marianela; Härtel, Steffen; Hermoso Ramello, Marcela; Leyton, Cecilia; González, Ma (2011)
      Objective Oral and ocular dryness are frequent and serious symptoms of Sjögren's syndrome (SS) that reflect problems in secretion due to glandular dysfunction. Exocytosis, an important process in the secretory pathway, ...
    • Chile's national center for health information systems: A public-private partnership to foster health care information interoperability 

      Capurro, Daniel; Echeverry, Aisen; Figueroa, Rosa; Guiñez, Sergio; Taramasco, Carla; Galindo, César; Avendaño, Angélica; García, Alejandra; Hartel, Steffen (IOS, 2017)
      Despite the continuous technical advancements around health information standards, a critical component to their widespread adoption involves political agreement between a diverse set of stakeholders. Countries that ...
    • Daam1a mediates asymmetric habenular morphogenesis by regulating dendritic and axonal outgrowth 

      Colombo, Alicia; Palma, Karina; Armijo, Lorena; Mione, Marina; Signore, Iskra A.; Morales, Camila; Guerrero, Néstor; Meynard, Margarita M.; Pérez, Ramón; Suazo, José; Marcelain Cubillos, Katherine; Briones, Luis; Härtel, Steffen; Wilson, Stephen W.; Concha Nordemann, Miguel (The Company of Biologists, 2013)
      Although progress has been made in resolving the genetic pathways that specify neuronal asymmetries in the brain, little is known about genes that mediate the development of structural asymmetries between neurons on left ...
    • Dendritic assembly of heteromeric γ-aminobutyric acid type B receptor subunits in hippocampal neurons 

      Ramírez, Omar A.; Vidal, René L.; Tello, Judith A.; Vargas, Karina J.; Kindler, Stefan; Härtel, Steffen; Couve, Andrés (2009)
      Understanding the mechanisms that control synaptic efficacy through the availability of neurotransmitter receptors depends on uncovering their specific intracellular trafficking routes. γ-Aminobutyric acid type B (GABAB) ...
    • Directional asymmetry in the volume of the human habenula 

      Ahumada Galleguillos, Patricio; Lemus, Carmen G.; Díaz, Eugenia; Osorio-Reich, María; Härtel, Steffen; Concha Nordemann, Miguel (Springer Verlag, 2017)
      © 2016, Springer-Verlag Berlin Heidelberg.Brain asymmetry is a conserved feature in vertebrates. The dorsal diencephalic habenular complex shows conspicuous structural and functional asymmetries in a wide range of species, ...
    • Evaluating Online Consumer Medication Information Systems: Comparative Online Usability Study 

      Sigle, Stefan; Barriga, Pilar; Correa Fernández, Francisco Javier; Juhra, Christian; Härtel, Steffen; Fegeler, Christian (JMIR, 2020)
      Background: Medication is the most common intervention in health care, and the number of online consumer information systems within the pharmaceutical sector is increasing. However, online consumer information systems can ...
    • Exercise and tachycardia increase NADPH oxidase and ryanodine receptor-2 activity: Possible role in cardioprotection 

      Sánchez, Gina; Escobar, Matías; Pedrozo Cibils, Zully; Macho Fisher, Pilar; Domenech Lira, Raúl; Härtel, Steffen; Hidalgo Tapia, María Cecilia; Donoso Laurent, Paulina (2008)
      Aim: Our objective was to investigate in cardiac muscle the contribution of NADPH oxidase to (a) ryanodine receptor-2 (RyR2) S-glutathionylation and (b) the preconditioning effects of exercise and tachycardia on infarct ...
    • Extra-embryonic tissue spreading directs early embryo morphogenesis in killifish 

      Reig Cardarella, Germán; Cerda Villablanca, Mauricio; Sepúlveda Salazar, Néstor; Flores Campo, Daniela; Castañeda, Víctor; Masazumi, Tada; Härtel, Steffen; Concha Nordemann, Miguel (Nature Publishing Group, 2017-06-05)
      The spreading of mesenchymal-like cell layers is critical for embryo morphogenesis and tissue repair, yet we know little of this process in vivo. Here we take advantage of unique developmental features of the non-conventional ...
    • Gold-standard for computer-assisted morphological sperm analysis 

      Chang, Violeta; García, Alejandra; Hitschfeld Kahler, Nancy; Härtel, Steffen (Elsevier, 2017)
      Background and Objective: Published algorithms for classification of human sperm heads are based on relatively small image databases that are not open to the public, and thus no direct comparison is available for competing ...
    • High-Frequency Field Stimulation of Primary Neurons Enhances Ryanodine Receptor-Mediated Ca2+ Release and Generates Hydrogen Peroxide, Which Jointly Stimulate NF-kB Activity 

      Riquelme, Denise; Álvarez, Alvaro; Leal, Nancy; Adasme, Tatiana; Espinoza, Italo; Valdés, Juan Antonio; Troncoso, Natalia; Härtel, Steffen; Hidalgo Tapia, Jorge; Hidalgo Tapia, María Cecilia; Carrasco Friz, María Angélica (2011)
      Neuronal electrical activity increases intracellular Ca2+ concentration and generates reactive oxygen species. Here, we show that high frequency field stimulation of primary hippocampal neurons generated Ca2+ signals with ...
    • Interfering of the Reelin/ApoER2/PSD95 Signaling Axis Reactivates Dendritogenesis of Mature Hippocampal Neurons 

      Ampuero, Estibaliz; Jury, Nur; Härtel, Steffen; Marzolo, María Paz; van Zundert, Brigitte (Wiley-Liss Inc., 2017)
      © 2016 Wiley Periodicals, Inc. Reelin, an extracellular glycoprotein secreted in embryonic and adult brain, participates in neuronal migration and neuronal plasticity. Extensive evidence shows that reelin via activation ...
    • Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration 

      Richardson, Jo; Gauert, Anton; Briones Montecinos, Luis; Kabla, Alexandre; Härtel, Steffen; Linker, Claudia (Cell Press, 2016)
      Collective cell migration is fundamental for life and a hallmark of cancer. Neural crest (NC) cells migrate collectively, but the mechanisms governing this process remain controversial. Previous analyses in Xenopus indicate ...