Now showing items 1-20 of 26

    • An oxidative stress-mediated positive-feedback iron uptake loop in neuronal cells 

      Núñez Millacura, Claudia; Tapia, Victoria; Muñoz, Patricia; Maccioni Baraona, Ricardo; Núñez González, Marco (2002)
      Intracellular reactive iron is a source of free radicals and a possible cause of cell damage. In this study, we analyzed the changes in iron homeostasis generated by iron accumulation in neuroblastoma (N2A) cells and ...
    • Antioxidant responses of cortex neurons to iron loading 

      Aguirre, Pabla; Mena, Natalia P.; Tapia, Victoria; Rojas, Alejandro; Arredondo Olguín, Miguel Armando; Núñez González, Marco (2006)
      Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. ...
    • Apical distribution of HFE-β2-microglobulin is associated with inhibition of apical iron uptake in intestinal epithelia cells 

      Arredondo, Miguel; Tapia, Victoria; Rojas, Alejandro; Aguirre, Pabla; Reyes, Francisca; Marzolo, Maria Paz; Núñez González, Marco (2006)
      Mutations in the HFE gene result in hereditary hemochromatosis, a disorder of iron metabolism characterized by increased intestinal iron absorption. Based on the observation that ectopic expression of HFE strongly inhibits ...
    • Apical distribution of HFE–b2-microglobulin is associated with inhibition of apical iron uptake in intestinal epithelia cells 

      Arredondo Olguín, Miguel Armando; Tapia, Victoria; Rojas, Alejandro; Aguirre, Pabla; Reyes, Francisca; Marzolo Canales, María Paz; Núñez González, Marco (SPRINGER, 2005-10-03)
      Mutations in the HFE gene result in hereditary hemochromatosis, a disorder of iron metabolism characterized by increased intestinal iron absorption. Based on the observation that ectopic expression of HFE strongly inhibits ...
    • Apotransferrin and holotransferrin undergo different endocytic cycles in intestinal epithelia (Caco-2) cells 

      Núñez González, Marco; Núñez-Millacura, Claudia; Beltrán, Marianela; Tapia, Victoria; Alvarez Hernández, Xavier (1997)
      Previous studies have demonstrated that diferric transferrin and apotransferrin compete for the binding to basolateral transfertin receptors and that transferrin-mediated iron uptake by Caco-2 cells is inhibited by ...
    • Cell death induced by mitochondrial complex I inhibition is mediated by Iron Regulatory Protein 

      Urrutia, Pamela J.; Aguirre, Pabla; Tapia, Victoria; Carrasco, Carlos M.; Mena, Natalia P.; Núñez González, Marco (Elsevier, 2017)
      Mitochondrial dysfunction and oxidative damage, often accompanied by elevated intracellular iron levels, are pathophysiological features in a number of neurodegenerative processes. The question arises as to whether ...
    • Cell death induced by mitochondrial complex I inhibition is mediated by Iron Regulatory Protein 1 

      Urrutia, Pamela J.; Aguirre, Pabla; Tapia, Victoria; Carrasco, Carlos M.; Mena, Natalia P.; Núñez González, Marco (Elsevier B.V., 2017)
      © 2017 Elsevier B.V. Mitochondrial dysfunction and oxidative damage, often accompanied by elevated intracellular iron levels, are pathophysiological features in a number of neurodegenerative processes. The question arises ...
    • Endocytic pathway of exogenous iron-loaded ferritin in intestinal epithelial (Caco-2) cells 

      Antileo, Elmer; Garri, Carolina; Tapia, Victoria; Muñoz, Juan Pablo; Chiong Lay, Mario; Nualart, Francisco; Lavandero González, Sergio; Díaz Fernández, Juan Cristóbal; Núñez González, Marco (American Physiological Society, 2013)
      Ferritin, a food constituent of animal and vegetal origin, is a source of dietary iron. Its hollow central cavity has the capacity to store up to 4,500 atoms of iron, so its potential as an iron donor is advantageous to ...
    • Hepcidin inhibits apical iron uptake in intestinal cells 

      Mena, Natalia P.; Esparza, Andrés; Tapia, Victoria; Valdés, Pamela; Núñez González, Marco (2007)
      Hepcidin (Hepc) is considered a key mediator in iron trafficking. Although the mechanism of Hepc action in macrophages is fairly well established, much less is known about its action in intestinal cells, one of the main ...
    • Inflammation alters the expression of DMT1, FPN1 and hepcidin, and it causes iron accumulation in central nervous system cells 

      Urrutia, Pamela J.; Aguirre, Pabla; Esparza, Andrés; Tapia, Victoria; Mena, Natalia P.; Arredondo Olguín, Miguel Armando; González Billault, Christian; Núñez González, Marco (2013)
      Inflammation and iron accumulation are present in a variety of neurodegenerative diseases that include Alzheimer’s disease and Parkinson’s disease. The study of the putative association between inflammation and iron ...
    • Intestinal epithelia (Caco-2) cells acquire iron through the basolateral endocytosis of transferrin 

      Núñez González, Marco; Tapia, Victoria; Arredondo, Miguel (1996)
      Although the absorption of iron through the intestinal epithelia is inversely related to body iron stores, the mechanisms by which the enterocytes sense body iron stores are unknown. Polarized enterocytes have transferrin ...
    • Iron homeostasis in neuronal cells: A role for IREG1 

      Aguirre, Pabla; Mena, Natalia; Tapia, Victoria; Arredondo, Miguel; Núñez González, Marco (2005)
      Background: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the ...
    • Iron homeostasis in neuronal cells: a role for IREGI 

      Aguirre, Pabla; Mena, Natalia P.; Tapia, Victoria; Arredondo Olguín, Miguel Armando; Núñez González, Marco (BIOMED CENTRAL LTD, 2005-01-24)
      Background: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the ...
    • Iron supply determines apical/basolateral membrane distribution of intestinal iron transporters DMT1 and ferroportin 1 

      Núñez González, Marco; Tapia, Victoria; Rojas, Alejandro; Aguirre, Pabla; Gómez, Francisco; Nualart, Francisco (2010)
      00168.2009.—Intestinal iron absorption comprises the coordinated activity of the influx transporter divalent metal transporter 1 (DMT1) and the efflux transporter ferroportin (FPN). In this work, we studied the movement ...
    • Iron toxicity in neurodegeneration 

      Núñez González, Marco; Urrutia, Pamela J.; Mena, Natalia; Aguirre, Pabla; Tapia, Victoria; Salazar, Julio (2012)
      Iron is an essential element for life on earth, participating in a plethora of cellular processes where one-electron transfer reactions are required. Its essentiality, coupled to its scarcity in aqueous oxidative environments, ...
    • Iron-induced oxidative damage in colon carcinoma (Caco-2) cells 

      Núñez González, Marco; Tapia, Victoria; Toyokuni, Shinya; Okada, Shigeru (Harwood Academic Publishers GmbH, 2001)
      Intestinal epithelial cells have an active apical iron uptake system that is involved in the regulated absorption of iron. By the action of this system, intestinal cells acquire increasing amounts of iron with time. Since ...
    • Iron-induced oxidative stress up-regulates calreticulin levels in intestinal epithelial (Caco-2) cells 

      Núñez González, Marco; Osorio, Andrea; Tapia, Victoria; Vergara, Alexander; Mura, Casilda V. (2001)
      Calreticulin, a molecular chaperone involved in the folding of endoplasmic reticulum synthesized proteins, is also a shock protein induced by heat, food deprivation, and chemical stress. Mobilferrin, a cytosolic isoform ...
    • Mathematical modeling of the dynamic storage of iron in ferritin 

      Salgado, J. Cristián; Olivera Nappa, Álvaro; Gerdtzen Hakim, Ziomara; Tapia, Victoria; Theil, Elizabeth C.; Conca Rosende, Carlos; Núñez González, Marco (BIOMED CENTRAL LTD, 2010-11-03)
      Background: Iron is essential for the maintenance of basic cellular processes. In the regulation of its cellular levels, ferritin acts as the main intracellular iron storage protein. In this work we present a mathematical ...
    • Neuroprotective Effect of a New 7,8-Dihydroxycoumarin-Based Fe2+/Cu2+ Chelator in Cell and Animal Models of Parkinson’s Disease 

      Aguirre, Pabla; García Beltrán, Olimpo; Tapia, Victoria; Muñoz, Yorka; Cassels Niven, Bruce; Núñez González, Marco (American Chemical Society, 2017)
      © 2016 American Chemical Society.Disturbed iron homeostasis, often coupled to mitochondrial dysfunction, plays an important role in the progression of common neurodegenerative diseases such as Parkinson’s disease (PD). ...
    • Progressive iron accumulation induces a biphasic change in the glutathione content of neuroblastoma cells 

      Núñez González, Marco; Gallardo, Vivian; Muñoz, Patricia; Tapia, Victoria; Esparza, Andrés; Salazar, Julio; Speisky Cosoy, Hernán (PERGAMON-ELSEVIER SCIENCE LTD, 2004-10-01)
      Glutathione (GSH) constitutes the single most important antioxidant in neurons, whereas iron causes oxidative stress that leads to cell damage and death. Although GSH and iron produce opposite effects on redox cell status, ...