Browsing by Author "4b36ab3b-329a-44f1-93a5-4da7c1eaa871"
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Arbildua, José J.; Brunet, Juan E.; Jameson, David M.; López, Maribel; Nova, Esteban; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (2006)The goal of this work was to determine the binding properties and location of 4′,6-diamidino-2-phenylindole (DAPI) complexed with tubulin. Using fluorescence anisotropy, a dissociation constant of 5.2 ± 0.4 μM for the ...
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Cruz Vargas, Victor Ignacio; Brunet, Juan E.; Jameson, David M. (1991)The quenching by molecular oxygen of the fluorescence of several probes complexed to apohorseradish peroxidase has been studied by intensity and time-resolved fluorescence methods. The probes utilized include 1-anilino-8 ...
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Günther Sapunar, Germán; Jameson, David; Aguilar, Joao; Sánchez, Susana (Elsevier, 2018-05-01)In this article, we review the application of fluorescence correlation spectroscopy (FCS) methods to studies on live cells. We begin with a brief overview of the theory underlying FCS, highlighting the type of information ...
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Lasagna, Mauricio; Cruz Vargas, Victor Ignacio; Jameson, David M.; Brunet, Juan E. (1996)The environmentally sensitive fluorescent probes 6-propionyl-2-(N,N- dimethylamino)naphthalene (PRODAN) and 2'-(N,N-dimethylamino)-6-naphthoyl-4- trans-cyclohexanioc acid (DANCA) form complexes with the heme binding site ...
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Sánchez, Susana A.; Brunet, Juan E.; Jameson, David M.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (2004)The pathway for the in vitro equilibrium unfolding of the tubulin heterodimer by guanidinium chloride (GdmCl) has been studied using several spectroscopic techniques, specifically circular dichroism (CD), two-photon ...