Now showing items 1-19 of 19

    • A BK (Slo1) channel journey from molecule to physiology 

      Contreras, Gustavo F.; Castillo, Karen; Enrique, Nicolás; Carrasquel Ursulaez, Willy; Castillo, Juan Pablo; Milesi, Verónica; Neely, Alan; Alvarez, Osvaldo; Ferreira, Gonzalo; Gonzalez, Carlos; Latorre, Ramón (Taylor and Francis Inc., 2013)
      Calcium and voltage-activated potassium (BK) channels are key actors in cell physiology, both in neuronal and non-neuronal cells and tissues. Through negative feedback between intracellular Ca2+ and membrane voltage, BK ...
    • A three-barrier model for the hemocyanin channel 

      Cecchi, Ximena; Alvarez, Osvaldo; Latorre, Ramón (1981)
      Keyhole limpet hemocyanin forms ion-conducting channels in planar lipid bilayer membranes. Ionic current through the open hemocyanin channel presents the following characteristics: (a) it is carried mainly by cations; (b) ...
    • Activation by divalent cations of a Ca2+-activated K+ channel from skeletal muscle membrane 

      Oberhauser, Andres; Alvarez, Osvaldo; Latorre, Ramón (1988)
      Several divalent cations were studied as agonists of a Ca2+-activated K+ channel obtained from rat muscle membranes and incorporated into planar lipid bilayers. The effect of these agonists on single-channel currents was ...
    • Cation permeability of a cloned rat epithelial amiloride-sensitive Na+ channel 

      Ismailov, Iskander I.; Shlyonsky, Vadim Gh; Alvarez, Osvaldo; Benos, Dale J. (1997)
      1. Conductance of heterotrimeric rat epithelial Na+ channels (α,β,γ-rENaCs) for Li+ and Na+ in planar lipid bilayers was a non-linear function of ion concentration, with a maximum of 30.4 ± 2.9 pS and 18.5 ± 1.9 pS at 1 M ...
    • Determination of the stoichiometry between alpha- and gamma 1 subunits of the BK channel using LRET 

      Carrasquel Ursulaez, Willy; Alvarez, Osvaldo; Bezanilla, Francisco; Latorre, Ramón (Cell Press, 2018-06-05)
      Two families of accessory proteins, beta and gamma, modulate BK channel gating and pharmacology. Notably, in the absence of internal Ca2+, the gamma 1 subunit promotes a large shift of the BK conductance-voltage curve to ...
    • Determination of the Stoichiometry between α- and γ1 Subunits of the BK Channel Using LRET 

      Carrasquel Ursulaez, Willy; Alvarez, Osvaldo; Bezanilla, Francisco; Latorre, Ramón (Biophysical Society, 2018)
      © 2018 Biophysical Society Two families of accessory proteins, β and γ modulate BK channel gating and pharmacology. Notably, in the absence of internal Ca2+, the γ1 subunit promotes a large shift of the BK conductance-voltage ...
    • Free energy perturbation simulations of cation binding to valinomycin 

      Eisenman, George; Alvarez, Osvaldo; Aqvist, Johan (Kluwer Academic Publishers, 1992)
      Experimental values of the free energies of cation binding to the cyclic depsipeptide molecule, valinomycin, obtained from Pedersen-type salt extraction measurements, provide data against which it is possible to test the ...
    • Gating charge displacement in a monomeric voltage-gated proton (Hv1) channel 

      Carmona, Emerson M.; Peter Larsson, H.; Neely, Alan; Alvarez, Osvaldo; Latorre, Ramón; Gonzalez, Carlos (National Academy of Sciences, 2018)
      © 2018 National Academy of Sciences. All Rights Reserved. The voltage-gated proton (Hv1) channel, a voltage sensor and a conductive pore contained in one structural module, plays important roles in many physiological ...
    • Modulation of BK channel voltage gating by different auxiliary β subunits 

      Contreras, Gustavo F.; Neely, Alan; Alvarez, Osvaldo; Gonzalez, Carlos; Latorre, Ramón (2012)
      Calcium- and voltage-activated potassium channels (BK) are regulated by a multiplicity of signals. The prevailing view is that different BK gating mechanisms converge to determine channel opening and that these gating ...
    • Peptides as promoters of ion-permeable channels 

      Tosteson, Magdalena T.; Alvarez, Osvaldo; Tosteson, Daniel C. (1985)
    • Pore accessibility during C-type inactivation in Shaker K+ channels 

      Basso, Claudia; Labarca, Pedro; Stefani, Enrico; Alvarez, Osvaldo; Latorre, Ramón (1998)
      Shaker K+ channels inactivate through two distinct molecular mechanisms: N-type, which involves the N-terminal domain and C-type that appears to involve structural modifications at the external mouth of the channel. We ...
    • Probing the pore size of the hemocyanin channel 

      Cecchi, Ximena; Bull Simpfendorfer, Ricardo; Franzoy, Roberto; Coronado, Roberto; Alvarez, Osvaldo (1982)
      We have studied single-channel conductance for different monovalent cations and streaming potentials caused by osmotic gradients of non-electrolytes in hemocyanin-treated membranes. We have found that the smaller ion, which ...
    • S3b amino acid residues do not shuttle across the bilayer in voltage-dependent Shaker K+ channels 

      Gonzalez, Carlos; Morera, Francisco J.; Rosenmann, Eduardo; Alvarez, Osvaldo; Latorre, Ramón (2005)
      In voltage-dependent channels, positive charges contained within the S4 domain are the voltage-sensing elements. The "voltage-sensor paddle" gating mechanism proposed for the KvAP K+ channel has been the subject of intense ...
    • SH oxidation coordinates subunits of rat brain ryanodine receptor channels activated by calcium and ATP 

      Bull Simpfendorfer, Ricardo; Marengo, Juan José; Finkelstein Muñoz, José Pablo; Behrens Pellegrino, María Isabel; Álvarez, Osvaldo (American Physiological Society, 2003)
      Bull, Ricardo, Juan Jose´ Marengo, Jose´ Pablo Finkelstein, Marı´a Isabel Behrens, and Osvaldo Alvarez. SH oxidation coordinates subunits of rat brain ryanodine receptor channels activated by calcium and ATP. Am J ...
    • Simultaneous recording of analog signals, audio and video using a VCR 

      Alvarez, Osvaldo (1995)
      An interface to record analog signals from DC to 20 kHz on the stereo sound channels of video cassette recorders is described. This interface is an effective solution for the implementation of experiments requiring ...
    • Structure and function of channels and channelogs as studied by computational chemistry 

      Eisenman, George; Alvarez, Osvaldo (Springer-Verlag, 1991)
    • The syndromic deafness mutation G12R impairs fast and slow gating in Cx26 hemichannels 

      García, Isaac E.; Villanelo, Felipe; Contreras, Gustavo F.; Pupo, Amaury; Pinto, Bernardo I.; Contreras, Jorge E.; Pérez-Acle, Tomás; Alvarez, Osvaldo; Latorre, Ramón; Martínez, Agustín D.; González, Carlos (Rockefeller University Press, 2018)
      © 2018 García et al. Mutations in connexin 26 (Cx26) hemichannels can lead to syndromic deafness that affects the cochlea and skin. These mutations lead to gain-of-function hemichannel phenotypes by unknown molecular ...
    • Tonic and phasic receptor neurons in the vertebrate olfactory epithelium 

      Madrid, Rodolfo; Sanhueza, Magdalena; Alvarez, Osvaldo; Bacigalupo Vicuña, Juan (Biophysical Society, 2003)
      Olfactory receptor neurons (ORNs) respond to odorants with characteristic patterns of action potentials that are relevant for odor coding. Prolonged odorant exposures revealed three populations of dissociated toad ORNs, ...
    • Voltage-dependent conductance induced by hemocyanin in black lipid films 

      Alvarez, Osvaldo; Diaz, Eliecer; Latorre, Ramón (1975)
      When hemocyanin is added to a black lipid film, the conductance increases in discrete steps. For negative potentials the single step conductance is constant, but for positive potentials the step conductance appears to ...