Now showing items 1-6 of 6

    • A conducting state with properties of a slow inactivated state in a Shaker K+ channel mutant 

      Olcese, Riccardo; Sigg, Daniel; Latorre, Ramón; Bezanilla, Francisco; Stefani, Enrico (ROCKEFELLER UNIV PRESS, 2001-02)
      In Shaker K+ channel, the amino terminus deletion Delta6-46 removes fast inactivation (N-type) unmasking a slow inactivation process. In Shaker Delta6-46 (Sh-IR) background, two additional mutations (T449V-I470C) remove ...
    • Correlation between charge movement and ionic current during slow inactivation in Shaker K+ channels 

      Olcese, Riccardo; Latorre, Ramón; Toro, Ligia; Bezanilla, Francisco; Stefani, Enrico (ROCKEFELLER UNIV PRESS, 1997-11)
      Prolonged depolarization induces a slow inactivation process in some Ki channels. We have studied ionic and gating currents during long depolarizations in the mutant Shaker H4-Delta(6-46) K+ channel and in the nonconducting ...
    • Pore accessibility during C-type inactivation in Shaker K+ channels 

      Basso, Claudia; Labarca, Pedro; Stefani, Enrico; Alvarez, Osvaldo; Latorre, Ramón (1998)
      Shaker K+ channels inactivate through two distinct molecular mechanisms: N-type, which involves the N-terminal domain and C-type that appears to involve structural modifications at the external mouth of the channel. We ...
    • Role of the S4 segment in a voltage-dependent calcium-sensitive potassium (hSlo) channel 

      Díaz Cuevas, Laín; Meera, Pratap; Amigo, Julio; Stefani, Enrico; Álvarez Araya, Osvaldo; Toro, Ligia; Latorre, Ramón (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 1998-12-04)
      We investigated the role of individual charged residues of the S4 region of a MaxiK channel (hSlo) in channel gating, We measured macroscopic currents induced by wild type (WT) and point mutants of hSlo in inside-out ...
    • Structural Determinants in the Interaction of Shaker Inactivating Peptide and a Ca2+-Activated K+ Channel 

      Toro, Ligia; Ottolia, Michela; Stefani, Enrico; Latorre, Ramón (1994)
      Shaker B inactivating peptide (BP) binds to its receptor in maxi Kca channels obstructing the flow of ions through them. The interaction between Kca channels and BP mutants, with different net charge and hydrophobicity, ...
    • Voltage-controlled gating in a large conductance Ca2+-sensitive K(+)channel (hslo) 

      Stefani, Enrico; Ottolia, M.; Noceti, F.; Olcese, Riccardo; Wallner, Martín; Latorre, Ramón; Toro, Ligia (NATL ACAD SCIENCES, 1997-05-13)
      Large conductance calcium- and voltage-sensitive K+ (MaxiK) channels share properties of voltage- and ligand-gated ion channels. In voltage-gated channels, membrane depolarization promotes the displacement of charged ...