Browsing by Author "e9e00d68-4d1d-405a-bb00-1c0ac8543671"
Now showing items 21-40 of 54
-
Tellez, Rowena; Allende, Catherine C.; Allende, Jorge E. (1992)Polyglutamate analogs of folate and related compounds were tested as inhibitors of casein kinase II (CK II) obtained from Xenopus laevis. The inhibitory capacity of the pteroyl, 4-amino-10-methyl pteroyl (the methotrexate ...
-
Bustos, Victor H.; Marin, Oriano; Meggio, Flavio; Cesaro, Luca; Allende, Catherine C.; Allende, Jorge E.; Pinna, Lorenzo A. (2005)Protein kinase CK1 denotes a family of pleiotropic serine/threonine protein kinases implicated in a variety of cellular functions. Typically, CK1 acts as a 'phosphate-directed' kinase whose targeting is primed by a single ...
-
Maldonado, Edio; Cabrejos, María Eugenia; Banks, Lawrence; Allende, Jorge E. (2002)Previous studies have shown that the HPV-1 6 E7 protein interacts with TBP. This interaction was found to take place through residues in the carboxy terminal half of E7, mutation of which resulted in weaker transforming ...
-
Human-Xenopus chimeras of Gsα reveal a new region important for its activation of adenylyl cyclase Antonelli, Marcelo; Birnbaumer, Lutz; Allende, Jorge E.; Olate, Juan (1994)G proteins are heterotrimeric GTPases that play a key role in signal transduction. The α subunit of Gs bound to GTP is capable of activating adenylyl cyclase. The amino acid sequences derived from two X. laevis cDNA clones ...
-
Campagna, Michela; Budini, Mauricio; Arnoldi, Francesca; Desselberger, Ulrich; Allende, Jorge E.; Burrone, Oscar R. (2007)The rotavirus (RV) non-structural protein 5, NSP5, is encoded by the smallest of the 11 genomic segments and localizes in 'viroplasms', cytoplasmic inclusion bodies in which viral RNA replication and packaging take place. ...
-
Solari Illescas, Aldo; Gatica, Marta; Allende, Jorge E. (1977)Yeast transfer RNA specific for phenylalanine has been treated chemically to remove either one or two nucleotides of its 3′ terminus and has been injected into Xenopus laevis oocytes to test whether this RNA can be repaired ...
-
Kofoid, Eric C.; Knauber, Donna C.; Allende, Jorge E. (1979)The maturation of Xenopus laevis oocytes was studied in media free of added potassium salts. Under these conditions maturation could be triggered by 1 mM Mn2+ and La3+ and, to a lesser extent, by 2-4 mM Ca2+ and Mg2+. ...
-
Olate, Juan; Jordana, Xavier; Allende, Catherine C.; Allende, Jorge E. (1983)The adenylate cyclase activity of membranes of Xenopus laevis oocytes and follicle cells was affected by the presence of 2-chloro-10-(3-aminopropyl)phenothiazine (CAPP) and two other antipsychotic drugs, fluphenazine and ...
-
Tarragó, Adela; Monasterio Opazo, Octavio; Allende, Jorge E. (1970)The two major methionyl-tRNA species from wheat embryos and E. coli have been studied as regards their capacity to form a ternary complex with GTP and the ribosomal binding enzyme from both sources. Methionyl-tRNA1 from ...
-
Carvallo, Pilar; Allende, Jorge E. (1987)The structural specificity of the purified protein synthesis initiation factor 2 (eIF-2) from X. laevis ovary towards analogs of GTP and GDP was studied. The relative affinity of the structural analogs was measured by their ...
-
Allende, Jorge E.; Tarragó, Adela; Shatkin, Aaron J. (1978)Wheat protein synthesis elongation factor 1 was tested for binding to GTP analogs, including structures resembling "caps" that are present at the 5′-termini of most eukaryotic mRNAs. The interaction was assayed by determining ...
-
Coronado, Alfonso; Mardones, Elvira; Allende, Jorge E. (1963)
-
Allende, Jorge E.; Allende, Catherine C.; Gatica, Marta; Matamala, María (1964)The aminoacyl-RNA synthetases have been shown to carry out the following two-step reaction: 1. 1) amino acid + ATP + enzyme ⇌ aminoacyl-AMP-enzyme + PPi 2. 2) aminoacyl-AMP-enzyme + sRNA ⇌ aminoacyl-sRNA + AMP + enzyme. ...
-
Seelenfreund Hirsch, Daniela; Allende, Catherine C.; Tarragó, Adela; Allende, Jorge E. (Academic Pres Australia, 1981)Histone H2b phosphorylated by a cAMP dependent protein kinase was injected into Xenopus laevis oocytes and the hydrolysis of phosphate groups measured. Two hours after injection 60% of the (32p) histone radioactivity had ...
-
Olate, Juan; Jorquera, Hugo; Purcell, Patricia; Codina, Juan; Birnbaumer, Lutz; Allende, Jorge E. (1989)Xenopus laevis oocytes are cells ideally suited to the study of signal transduction and of the G-proteins that are involved in this process. A X. laevis cDNA library in λgt10 has been screened with a mixture of three ...
-
Olate, Juan; Martinez, Sixta; Purcell, Patricia; Jorquera, Hugo; Codina, Juan; Bimbaumer, Lutz; Allende, Jorge E. (Elsevier, 1990)A cDNA library preprared from Xenopus laevis oocytes in λgt10 was screened with a mixture of three oligonucleotide probes designed to detect sequences found in different mammalian genes coding for a-subunits of G-proteins. ...
-
Gatica, Marta; Allende, Catherine C.; Allende, Jorge E. (1989)Casein kinase II purified from nuclei of Xenopus laevis oocytes is inhibited by several specific nucleic acids. This kinase, the main phosphorylating activity of the oocyte nucleus, is markedly inhibited by poly U at 10 ...
-
Allende, Catherine C.; Hinrichs, M. Victoria; Santos, Eugenio; Allende, Jorge E. (1988)Microinjection of the activated ras oncogenic protein can induce the meiotic maturation of Xenopus laevis oocytes, a process that can also be triggered by progesterone or high concentrations of insulin. Cycloheximide and ...
-
Birnbaumer, Lutz; Olate, Juan; Allende, Catherine C.; Allende, Jorge E.; Sekura, Ronald D. (1984)Membranes were obtained from Xenopus laevis oocytes after removal of follicular cells by collagenase treatment. [32P]ADP-ribosylation with pertussis toxin showed them to contain a single Mr = 40 000 substrate for this toxin ...
-
Orellana Orellana, Omar; Jedlicki Corbeaux, Eugenia; Allende, Catherine; Allende Rivera, Jorge (Elsevier, 1984)A calmodulin-Ca2+-stimulated cyclic nucleotide phosphodiesterase (EC 3.1.4.17) which hydrolyzed both cGMP and cAMP has been purified about 2000-fold from ovaries of the amphibian Xenopus laevis. Gel filtration through ...