Browsing by Author "Salgado, J. Cristián"

Now showing items 1-7 of 7

    • Current insights on protein behaviour in hydrophobic interaction chromatography 
      Lienqueo Contreras, María Elena; Mahn Osses, Andrea; Salgado, J. Cristián; Asenjo de Leuze, Juan (2007)
      This paper gives a summary of different aspects for predicting protein behaviour in hydrophobic interaction chromatography (HIC). First, a brief description of HIC, hydrophobic interactions, amino acid and protein ...
    • Effects of natural flavones and flavonols on the kinase activity of Cdk5 
      Zapata Torres, Gerald Amilcar; Opazo, Felipe; Salgado, J. Cristián; Muñoz Concha, Juan; Krautwurst, Hans; Mascayano, Carolina; Sepúlveda Boza, Silvia; Maccioni Baraona, Ricardo; Cassels Niven, Bruce (AMER CHEMICAL SOC, 2004-03)
      A number of natural and synthetic flavonoids have been assessed previously with regard to their effects on the activity of cyclin-dependent kinases (Cdk1 and -2) related to the inhibition of cell cycle progression. On the ...
    • Is it possible to predict the average surface hydrophobicity of a protein using only its amino acid composition? 
      Rapaport Zimermann, Iván; Salgado, J. Cristián; Asenjo de Leuze, Juan (ELSEVIER SCIENCE BV, 2005-05-20)
      Hydrophobicity is one of the most important physicochemical properties of proteins. Moreover, it plays a fundamental role in hydrophobic interaction chromatography, a separation technique that, at present time, is used in ...
    • Mathematical modeling of the dynamic storage of iron in ferritin 
      Salgado, J. Cristián; Olivera Nappa, Álvaro; Gerdtzen Hakim, Ziomara; Tapia, Victoria; Theil, Elizabeth C.; Conca Rosende, Carlos; Núñez González, Marco (BIOMED CENTRAL LTD, 2010-11-03)
      Background: Iron is essential for the maintenance of basic cellular processes. In the regulation of its cellular levels, ferritin acts as the main intracellular iron storage protein. In this work we present a mathematical ...
    • New approaches for predicting protein retention time in hydrophobic interaction chromatography 
      Lienqueo Contreras, María Elena; Mahn Osses, Andrea; Navarro, Gonzalo; Salgado, J. Cristián; Pérez-Acle, Tomás; Rapaport Zimermann, Iván; Asenjo de Leuze, Juan (JOHN WILEY, 2006-08)
      Hydrophobic interaction chromatography (HIC) is an important technique for the purification of proteins. In this paper, we review three different approaches for predicting protein retention time in HIC, based either on a ...
    • Predicting the behaviour of proteins in hydrophobic interaction chromatography 1: Using the hydrophobic ... 
      Salgado, J. Cristián; Rapaport Zimermann, Iván; Asenjo de Leuze, Juan (ELSEVIER, 2006-02-24)
      This paper focuses on the prediction of the dimensionless retention time of proteins (DRT) in hydrophobic interaction chromatography (HIC) by means of mathematical models based on characteristics of the surface hydrophobicity ...
    • Predicting the behaviour of proteins in hydrophobic interaction chromatography 2. Using a statistical ... 
      Salgado, J. Cristián; Rapaport Zimermann, Iván; Asenjo de Leuze, Juan (ELSEVIER, 2006-02-24)
      This paper focuses on the prediction of the dimensionless retention time (DRT) of proteins in hydrophobic interaction chromatography (HIC) by means of mathematical models based on the statistical description of the amino ...