Browsing by Author "201-0281205"
Now showing items 21-29 of 29
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Stellwagen, Earle; Babul Cattán, Jorge (1975)Increasing concentrations of chloride were found to increase the resolution between two visible absorbance spectral transitions associated with acidification of ferricytochrome c. Analysis of a variety of spectral and ...
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Báez, Mauricio; Rodríguez, Patricio H.; Babul Cattán, Jorge; Guixé Leguía, Victoria Cristina (2003)Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with pyrene maleimide (PM) results in a rapid inactivation of the enzyme. The loss of enzyme activity correlates with the incorporation of 2 mol of PM/mol of ...
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Cabrera Paucar, Ricardo; Báez, Mauricio; Pereira, Humberto M.; Caniuguir, Andrés; Garratt, Richard C.; Babul Cattán, Jorge (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2011-02-18)Substrate inhibition by ATP is a regulatory feature of the phosphofructokinases isoenzymes from Escherichia coli (Pfk-1 and Pfk-2). Under gluconeogenic conditions, the loss of this regulation in Pfk-2 causes substrate ...
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Cabrera Paucar, Ricardo; Báez, Mauricio; Pereira, Humberto M.; Caniuguir, André S.; Garratt, Richard C.; Babul Cattán, Jorge (2011)Substrate inhibition by ATP is a regulatory feature of the phosphofructokinases isoenzymes from Escherichia coli (Pfk-1 and Pfk-2). Under gluconeogenic conditions, the loss of this regulation in Pfk-2 causes substrate ...
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Ramírez Sarmiento, César A.; Báez, Mauricio; Zamora, Ricardo A.; Balasubramaniam, Deepa; Babul Cattán, Jorge; Komives, Elizabeth; Guixé Leguía, Victoria Cristina (Biophysical Society, 2015)© 2015 Biophysical Society. Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highly cooperative three-state folding mechanism N<inf>2</inf> → 2I → 2U. The strong coupling ...
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Medina, Exequiel; Córdova, Cristóbal; Villalobos, Pablo; Reyes, Javiera; Komives, Elizabeth A.; Ramírez Sarmiento, César A.; Babul Cattán, Jorge (Cell Press, 2016)The forkhead family of transcription factors (Fox) controls gene transcription during key processes such as regulation of metabolism, embryogenesis, and immunity. Structurally, Fox proteins feature a conserved DNA-binding ...
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Báez, Mauricio; Merino, Felipe; Astorga, Guadalupe; Babul Cattán, Jorge (ELSEVIER, 2008-06-11)Binding of MgATP to an allosteric site of Escherichia coli phosphofructokinase-2 (Pfk-2) provoked inhibition and a dimer-tetramer (D-T) conversion of the enzyme. Successive deletions of up to 10 residues and point mutations ...
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Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli Báez, Mauricio; Cabrera Paucar, Ricardo; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (2007)Escherichia coli phosphofructokinase-2 (Pfk-2) is an oligomeric enzyme characterized by two kinds of interfaces: a monomer-monomer interface, critical for enzymatic activity, and a dimer-dimer interface formed upon ...
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Carvajal, Alonso I.; Vallejos, Gabriel; Komives, Elizabeth A.; Castro Fernández, Víctor; Leonardo, Diego A.; Garratt, Richard C.; Ramírez Sarmiento, César A.; Babul Cattán, Jorge (Wiley, 2017)Cold shock proteins (Csp) constitute a family of ubiquitous small proteins that act as RNA-chaperones to avoid cold-induced termination of translation. All members contain two subdomains composed of 2 and 3 beta-strands, ...