Browsing by Author "Jameson, David M."

Now showing items 1-3 of 3

    • Fluorescence resonance energy transfer and molecular modeling studies on 4 ',6-diamidino-2-phenylindole ... 
      Arbildua, José J.; Brunet, Juan E.; Jameson, David M.; López, Maribel; Nova Martínez, Esteban; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (COLD SPRING HARBOR LAB PRESS, 2006-03)
      The goal of this work was to determine the binding properties and location of 4',6-diamidino-2-phenyl=indole (DAPI) complexed with tubulin. Using fluorescence anisotropy, a dissociation constant of 5.2 +/- 0.4 mu M for the ...
    • Single tryptophan mutants of FtsZ: Nucleotide binding/exchange and conformational transitions 
      Montecinos Franjola, Felipe Antonio; James, Nicholas G.; Concha Marambio, Luis; Brunet, Juan E.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio; Jameson, David M. (Elsevier, 2014)
      Cell division protein FtsZ cooperatively self-assembles into straight filaments when bound to GTP. A set of conformational changes that are linked to FtsZ GTPase activity are involved in the transition from straight to ...
    • Studies on the Dissociation and Urea-Induced Unfolding of FtsZ Support the Dimer Nucleus Polymerization Mechanism 
      Montecinos Franjola, Felipe; Ross, Justin A.; Sánchez, Susana A.; Brunet, Juan E.; Lagos Mónaco, Rosalba; Jameson, David M.; Monasterio Opazo, Octavio (2012-05)
      FtsZ is a major protein in bacterial cytokinesis that polymerizes into single filaments. A dimer has been proposed to be the nucleating species in FtsZ polymerization. To investigate the influence of the self-assembly of ...