Browsing by Author "Lobos, S."
Now showing items 1-16 of 16
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Lobos, S.; Mora, Guido C. (VCH PUBLISHERS INC, 1991-06)Studies on Salmonella typhi and Salmonella typhimurium outer membrane proteins have shown that the relative position of OmpC porin in sodium dodecyl sulfate-polyacrylamide gel electrophoresis undergoes an important shift ...
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Gutiérrez Mostafá, Matías Ricardo; Rojas Soto, Luis; Mancilla Villalobos, Rodrigo; Seelenfreund Hirsch, Daniela; Vicuña E., Rafael; Lobos, S. (SPRINGER, 2008-10)In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn2+ homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been ...
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Calderón, Inés; Lobos, S.; Rojas, Héctor A.; Palomino, Carmen; Rodríguez, Luis H.; Mora, Guido C. (American Society for Microbiology, 1986)Immunoglobulin G (IgC)-and IgM-specific antibody titers against Salmonella typhi Ty2 porins have been measured in 30 paired typhoid sera by enzyme-linked immunosorbent assay. These studies have found that IgG serum titers. ...
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Polanco, R.; Lobos, S.; Vicuña E., Rafael (ELSEVIER SCIENCE INC, 2002-04-16)The white rot basidiomycete Ceriporiopsis subvermispora secretes the ligninolytic enzymes manganese-dependent peroxidase (MnP) and laccase to the extracellular medium. The promoter region of the laccase gene (Cs-lcsl) ...
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Lobos, S.; Larrondo, F.; Salas, L.; Karahanian, Eduardo; Vicuña E., Rafael (ELSEVIER SCIENCE BV, 1998-01-12)A cDNA (MnP13-1) and the Cs-mnp1 gene encoding for an isoenzyme of manganese peroxidase (MnP) from C. subvermispora were isolated separately and sequenced. The cDNA, identified in a library constructed in the vector Lambda ...
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Manubens, Augusto; Lobos, S.; Jadue, Yael; Toro, Manuel; Messina, Rosa; Lladser Prado, Manuel; Seelenfreund Hirsch, Daniela (Kluwr Academic Publisher, 1999)Traditional identification of peach and nectarine varieties relies on the assessment of agronomic traits of the adult plant. This leads to a significant delay of time, constraints to breeders in the surveillance of germplasm ...
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Lobos, S.; Tello, Mario; Polanco, R.; Larrondo, L. F.; Manubens, Augusto; Salas, L.; Vicuña E., Rafael (CURRENT SCIENCE ASSN, 2001-10-25)Lignin, the most abundant renewable source of aromatic carbon on earth, consists in a highly irregular three-dimensional biopolymer of oxygenated phenylpropanoid units. In natural environments, lignin is only degraded ...
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Cárcamo, J.; Lobos, S.; Merino, A.; Buckbinder, L.; Weinmann, R.; Natarajan, R.; Reinberg, D. (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC,, 2010-11-16)The role of the adenovirus major late upstream transcription from the adenovirus major late and the IV a2 promoters was studied. The transcription initiation site of the IVa2 promoter is located 210 nucleotides upstream ...
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Calderón, Inés; Lobos, S.; Mora, Guido C. (Springer-Verlag, 1984)Two outer membrane proteins of Salmonella typhi T y 2 were extensively co-purified. According to their migration in dodecyl sulfate/polyacrylamide gel electrophoresis and solubility characteristics, these proteins are ...
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Larrondo, L. F.; Lobos, S.; Stewart, P.; Cullen, D.; Vicuña E., Rafael (AMER SOC MICROBIOLOGY, 2001-05)We expressed cDNAs coding for manganese peroxidases (MnPs! from the basidiomycetes Ceriporiopsis subvermispora (MnP1) and Phanerochaete chrysosporium (H4) under control of the alpha -amylase promoter from. Aspergillus ...
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Rajakumar, S.; Gaskell, J.; Cullen, C.; Lobos, S.; Karahanian, Eduardo; Vicuña E., Rafael (AMER SOC MICROBIOLOGY, 1996-07)Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described ...
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Canales, Mauricio; Lobos, S.; Vicuña, Rafael (Universidad Católica de Valparaíso, 1998-08-15)Ceriporiopsis subvermisporais a white-rot basidiomycete that produces several isoenzymes of manganese peroxidase (MnP). A cADN of one of them (MnP13-1) has been isolated and sequenced. The deduced aminoacid sequence ...
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Labarca, Pedro; Lobos, S.; Calderón, Inés; Mora, Guido C. (Elsevier Science Publisher B. V., 1986)Native porins from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins ...
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Salas, Carlos; Lobos, S.; Larraín, Juan; Salas, Loreto; Cullen, D.; Vicuña E., Rafael (PORTLAND PRESS, 1995-06)Laccase is one of the ligninolytic enzymes found in liquid cultures of the fungus Ceriporiopsis subvermispora in defined medium. As an approach to a clarification of the role of laccases during the attack on lignin by the ...
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Karahanian, Eduardo; Corsini, Gino; Lobos, S.; Vicuña E., Rafael (ELSEVIER SCIENCE BV, 1998-11-26)A gene encoding laccase has been isolated from a genomic library of the white-rot basidiomycete Ceriporiopsis subvermispora constructed in Lambda GEM-ii. This gene (Cs-lcs1) contains an open reading frame of 2215 bp, ...
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Cárcamo, J.; Lobos, S.; Merino, A.; Buckbinder, L.; Wienmann, R.; Natarajan, V.; Reinberg, D. (National Institute of Health, 1989)Almost every week, it seems, another character appears, expending the cast of DNA-binding proteins. Some of these proteins are destined for major roles, binding to DNA sequences a few hundred basepairs upstream of key ...