Disease Resistance in Atlantic Salmon (Salmo salar): Coinfection of the Intracellular Bacterial Pathogen Piscirickettsia salmonis and the Sea Louse Caligus rogercresseyi
Author
dc.contributor.author
Lhorente, Jean Paul
Author
dc.contributor.author
Gallardo, José A.
es_CL
Author
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Villanueva, Beatriz
es_CL
Author
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Carabaño, María J.
es_CL
Author
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Neira Roa, Roberto
es_CL
Admission date
dc.date.accessioned
2014-12-19T14:48:30Z
Available date
dc.date.available
2014-12-19T14:48:30Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
Plos One April 2014 | Volume 9 | Issue 4 | e95397
en_US
Identifier
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DOI: 10.1371/journal.pone.0095397
Identifier
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https://repositorio.uchile.cl/handle/2250/120255
General note
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Artículo de publicación ISI
en_US
Abstract
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Background: Naturally occurring coinfections of pathogens have been reported in salmonids, but their consequences on
disease resistance are unclear. We hypothesized that 1) coinfection of Caligus rogercresseyi reduces the resistance of Atlantic
salmon to Piscirickettsia salmonis; and 2) coinfection resistance is a heritable trait that does not correlate with resistance to a
single infection.
Methodology: In total, 1,634 pedigreed Atlantic salmon were exposed to a single infection (SI) of P. salmonis (primary
pathogen) or coinfection with C. rogercresseyi (secondary pathogen). Low and high level of coinfection were evaluated
(LC = 44 copepodites per fish; HC = 88 copepodites per fish). Survival and quantitative genetic analyses were performed to
determine the resistance to the single infection and coinfections.
Main Findings: C. rogercresseyi significantly increased the mortality in fish infected with P. salmonis (SI mortality = 251/545;
LC mortality = 544/544 and HC mortality = 545/545). Heritability estimates for resistance to P. salmonis were similar and of
medium magnitude in all treatments (h2
SI = 0.2360.07; h2
LC = 0.1760.08; h2
HC = 0.2460.07). A large and significant genetic
correlation with regard to resistance was observed between coinfection treatments (rg LC-HC = 0.9960.01) but not between
the single and coinfection treatments (rg SI-LC =20.1460.33; rg SI-HC = 0.3260.34).
Conclusions/Significance: C. rogercresseyi, as a secondary pathogen, reduces the resistance of Atlantic salmon to the
pathogen P. salmonis. Resistance to coinfection of Piscirickettsia salmonis and Caligus rogercresseyi in Atlantic salmon is a
heritable trait. The absence of a genetic correlation between resistance to a single infection and resistance to coinfection
indicates that different genes control these processes. Coinfection of different pathogens and resistance to coinfection
needs to be considered in future research on salmon farming, selective breeding and conservation.
en_US
Patrocinador
dc.description.sponsorship
This study was financed by INNOVA-CHILE of CORFO through the projects: 1) Consorcio empresarial de gene´tica y desarrollo biotecnolo´ gico para la
industria salmonera (Nu 206-5047), and 2) Desarrollo de una nueva metodologı´a para la identificacio´n y seleccio´n de salmo´ nidos gene´ticamente resistentes al
ectopara´sito Caligus rogercresseyi (07CN13PBT-61). This study is a collaborative work of different institutions belonging to ‘‘Red de gene´ tica e inmunologı´a para el
control de pato´genos en Acuicultura’’ (RED CYTED Nu 11RT0420). BV received funding from the Ministerio de Economı´a y Competitividad, Spain (project CGL2012-
39861-C02-02).
Disease Resistance in Atlantic Salmon (Salmo salar): Coinfection of the Intracellular Bacterial Pathogen Piscirickettsia salmonis and the Sea Louse Caligus rogercresseyi