Author | dc.contributor.author | Vicencio, José Miguel | |
Author | dc.contributor.author | Ortíz, C. | es_CL |
Author | dc.contributor.author | Criollo Céspedes, Alfredo | es_CL |
Author | dc.contributor.author | Jones, A. W. E. | es_CL |
Author | dc.contributor.author | Kepp, O. | es_CL |
Author | dc.contributor.author | Galluzzi, Lorenzo | es_CL |
Author | dc.contributor.author | Joza, N. | es_CL |
Author | dc.contributor.author | Vitale, I. | es_CL |
Author | dc.contributor.author | Morselli, E. | es_CL |
Author | dc.contributor.author | Tailler, M. | es_CL |
Author | dc.contributor.author | Castedo, M. | es_CL |
Author | dc.contributor.author | Maiuri, M. C. | es_CL |
Author | dc.contributor.author | Molgó, Jordi | es_CL |
Author | dc.contributor.author | Szabadkai, G. | es_CL |
Author | dc.contributor.author | Lavandero González, Sergio | es_CL |
Author | dc.contributor.author | Kroemer, Guido | es_CL |
Admission date | dc.date.accessioned | 2010-06-15T14:20:46Z | |
Available date | dc.date.available | 2010-06-15T14:20:46Z | |
Publication date | dc.date.issued | 2009-07 | |
Cita de ítem | dc.identifier.citation | CELL DEATH AND DIFFERENTIATION 16 (7): Special Issue: SI Pages: 1006-1017 | en_US |
Identifier | dc.identifier.issn | 1350-9047 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/121007 | |
Abstract | dc.description.abstract | The inositol 1,4,5-trisphosphate receptor (IP3R) is a major regulator of apoptotic signaling. Through interactions with members of the Bcl-2 family of proteins, it drives calcium (Ca2+) transients from the endoplasmic reticulum (ER) to mitochondria, thereby establishing a functional and physical link between these organelles. Importantly, the IP3R also regulates autophagy, and in particular, its inhibition/depletion strongly induces macroautophagy. Here, we show that the IP3R antagonist xestospongin B induces autophagy by disrupting a molecular complex formed by the IP3R and Beclin 1, an interaction that is increased or inhibited by overexpression or knockdown of Bcl-2, respectively. An effect of Beclin 1 on Ca2+ homeostasis was discarded as siRNA-mediated knockdown of Beclin 1 did not affect cytosolic or luminal ER Ca2+ levels. Xestospongin B- or starvation-induced autophagy was inhibited by overexpression of the IP3R ligand-binding domain, which coimmunoprecipitated with Beclin 1. These results identify IP3R as a new regulator of the Beclin 1 complex that may bridge signals converging on the ER and initial phagophore formation. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | NATURE PUBLISHING GROUP | en_US |
Keywords | dc.subject | Apoptosis | en_US |
Título | dc.title | The inositol 1,4,5-trisphosphate receptor regulates autophagy through its interaction with Beclin 1 | en_US |
Document type | dc.type | Artículo de revista | |