Monitoring bacterial consumption of low molecular weight lignin derivatives by high performance liquid chromatography
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The lignolytic capacity of some natural bacterial isolates eas examined. Strains were selected from samples of decaying wood by growth in a minimal medium containing aromatic compounds with a structural relationship to lignin as the sole carbon sources. These included derivatives of benzoic and phenylpropanoic acids, as well as a mixture of low molecular weight compounds obtained by fractionation of kraft lignin. Reserved-phase high-performnce liquid chromatography analyses before and after cell growth in the latter revealed a degradation pattern of the different compounds present in the culture which was characteristic for each of the strains studied.
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