DNA, Cell Wall and General Oxidative Damage Underlie the Tellurite/Cefotaxime Synergistic Effect in Escherichia coli
Author
dc.contributor.author
Molina Quiroz, Roberto C.
Author
dc.contributor.author
Loyola, David E.
es_CL
Author
dc.contributor.author
Muñoz Villagrán, Claudia
es_CL
Author
dc.contributor.author
Quatrini, Raquel
es_CL
Author
dc.contributor.author
Vásquez, Claudio C.
es_CL
Author
dc.contributor.author
Pérez Donoso, José
es_CL
Admission date
dc.date.accessioned
2014-01-08T17:05:47Z
Available date
dc.date.available
2014-01-08T17:05:47Z
Publication date
dc.date.issued
2013
Cita de ítem
dc.identifier.citation
PLOS ONE, November 2013, Volume 8, Isue 11, e79499
en_US
Identifier
dc.identifier.other
doi: 10.1371/journal.pone.0079499
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/121720
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
The constant emergence of antibiotic multi-resistant pathogens is a concern worldwide. An alternative for bacterial
treatment using nM concentrations of tellurite was recently proposed to boost antibiotic-toxicity and a synergistic effect of
tellurite/cefotaxime (CTX) was described. In this work, the molecular mechanism underlying this phenomenon is proposed.
Global changes of the transcriptional profile of Escherichia coli exposed to tellurite/CTX were determined by DNA
microarrays. Induction of a number of stress regulators (as SoxS), genes related to oxidative damage and membrane
transporters was observed. Accordingly, increased tellurite adsorption/uptake and oxidative injuries to proteins and DNA
were determined in cells exposed to the mixture of toxicants, suggesting that the tellurite-mediated CTX-potentiating effect
is dependent, at least in part, on oxidative stress. Thus, the synergistic tellurite-mediated CTX-potentiating effect depends
on increased tellurite uptake/adsorption which results in damage to proteins, DNA and probably other macromolecules.
Our findings represent a contribution to the current knowledge of bacterial physiology under antibiotic stress and can be of
great interest in the development of new antibiotic-potentiating strategies.