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The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the Chicken

Authordc.contributor.authorPezoa, David 
Authordc.contributor.authorYang, Hee Jeong es_CL
Authordc.contributor.authorBlondel, Carlos J. es_CL
Authordc.contributor.authorSantiviago Cid, Carlos es_CL
Authordc.contributor.authorAndrews Polymenis, Helene L. es_CL
Authordc.contributor.authorContreras, Inés es_CL
Admission datedc.date.accessioned2014-01-30T14:16:19Z
Available datedc.date.available2014-01-30T14:16:19Z
Publication datedc.date.issued2013
Cita de ítemdc.identifier.citationPLoS ONE 8(5): e63917en_US
Identifierdc.identifier.otherdoi:10.1371/ journal.pone.0063917
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/121799
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractThe role of the Salmonella Pathogenicity Islands (SPIs) in pathogenesis of Salmonella enterica Typhimurium infection in the chicken is poorly studied, while many studies have been completed in murine models. The Type VI Secretion System (T6SS) is a recently described protein secretion system in Gram-negative bacteria. The genus Salmonella contains five phylogenetically distinct T6SS encoded in differentially distributed genomic islands. S. Typhimurium harbors a T6SS encoded in SPI-6 (T6SSSPI-6), which contributes to the ability of Salmonella to colonize mice. On the other hand, serotype Gallinarum harbors a T6SS encoded in SPI-19 (T6SSSPI-19) that is required for colonization of chicks. In this work, we investigated the role of T6SSSPI-6 in infection of chicks by S. Typhimurium. Oral infection of White Leghorn chicks showed that a DT6SSSPI-6 mutant had reduced colonization of the gut and internal organs, compared with the wild-type strain. Transfer of the intact T6SSSPI-6 gene cluster into the T6SS mutant restored bacterial colonization. In addition, our results showed that transfer of T6SSSPI-19 from S. Gallinarum to the DT6SSSPI-6 mutant of S. Typhimurium not only complemented the colonization defect but also resulted in a transient increase in the colonization of the cecum and ileum of chicks at days 1 and 3 post-infection. Our data indicates that T6SSSPI-6 contributes to chicken colonization and suggests that both T6SSSPI-6 and T6SSSPI-19 perform similar functions in vivo despite belonging to different phylogenetic families.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherPLOS ONEen_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Títulodc.titleThe Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the Chickenen_US
Document typedc.typeArtículo de revistaen_US


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile