Author | dc.contributor.author | Speisky Cosoy, Hernán | |
Author | dc.contributor.author | Gómez, Maritza | es_CL |
Author | dc.contributor.author | Carrasco Pozo, Catalina | es_CL |
Author | dc.contributor.author | Pastene, Edgar | es_CL |
Author | dc.contributor.author | López Alarcón, Camilo | es_CL |
Author | dc.contributor.author | Olea Azar, Claudio | es_CL |
Admission date | dc.date.accessioned | 2010-01-19T19:25:35Z | |
Available date | dc.date.available | 2010-01-19T19:25:35Z | |
Publication date | dc.date.issued | 2008-07-01 | |
Cita de ítem | dc.identifier.citation | BIOORGANIC & MEDICINAL CHEMISTRY, Volume: 16, Issue: 13, Pages: 6568-6574, 2008 | en_US |
Identifier | dc.identifier.issn | 0968-0896 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/123927 | |
Abstract | dc.description.abstract | Cu2+ ions and GSH molecules interact to swiftly form the complex Cu(I)–glutathione. We investigated the
potential capacity of such complex to reduce molecular oxygen. The addition of SOD to a solution containing
Cu(I)–glutathione led to a sustained decline of the basal oxygen level. Such effect was partially
reverted by the addition of catalase. The complex was able to induce the reduction of cytochrome c
and the oxidation of dyhydroethidium into 2-hydroxyethidium. Both effects were totally blocked by
SOD. The ability of the complex to generate superoxide radicals was confirmed by EPR spin-trapping.
Cu(I)–glutathione induce no oxidation of fluorescein, a hydroxyl radical-sensitive probe. We conclude
that in solutions containing the complex, oxygen is continually reduced into superoxide, and that—in
absence of interceptors—the latter radicals are quantitatively re-oxidized into molecular oxygen. We suggest
that by functioning as a continuous source of superoxide, the complex could potentially affect a
broad range of susceptible biological targets. | en_US |
Patrocinador | dc.description.sponsorship | This work was supported by FONDECYT #1070613. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | en_US |
Keywords | dc.subject | Copper–glutathione complex | en_US |
Título | dc.title | Cu(I)–Glutathione complex: A potential source of superoxide radicals generation | en_US |
Document type | dc.type | Artículo de revista | |