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CCS and SOD1 mRNA are reduced after copper supplementation in peripheral mononuclear cells of individuals with high serum ceruloplasmin concentration

Authordc.contributor.authorSuazo Romo, Miriam 
Authordc.contributor.authorOlivares, Felipe es_CL
Authordc.contributor.authorMéndez, Marco es_CL
Authordc.contributor.authorPulgar, Rodrigo es_CL
Authordc.contributor.authorProhaska, Joseph R. es_CL
Authordc.contributor.authorArredondo Olguín, Miguel Armando es_CL
Authordc.contributor.authorPizarro Aguirre, Fernando es_CL
Authordc.contributor.authorOrdóñez Pizarro, Fernando es_CL
Authordc.contributor.authorAraya, Magdalena es_CL
Authordc.contributor.authorGonzález Canales, Mauricio 
Cita de ítemdc.identifier.citationJOURNAL OF NUTRITIONAL BIOCHEMISTRY, Volume: 19, Issue: 4, Pages: 269-274, 2008en_US
Abstractdc.description.abstractThe limits of copper homeostatic regulation in humans are not known, making it difficult to define the milder effects of early copper excess. Furthermore, a robust assay to facilitate the detection of early stages of copper excess is needed. To address these issues, we assessed changes in relative mRNA abundance of methallothionein 2A (MT2A), prion (PrP), amyloid precursor-like protein 2 (APLP2), Cu/Zn superoxide dismutase (SOD1) and its copper chaperone (CCS) in peripheral mononuclear cells (PMNCs) from healthy adults representing the 5% highest and lowest extremes in the distribution curve of serum ceruloplasmin (Cp) concentrations of 800 individuals. The intracellular Cu content was also determined. PMNCs were isolated from individuals before and after exposure to a single daily dose of 10 mg Cu (as CuSO4) for 2 months. Results showed that although there were fluctuations in serum Cp values of the samples assessed before copper exposure, no significant differences were observed in cell copper content or in the relative abundance of MT2A, PrP and APLP2 transcripts in PMNCs. Also, these values were not modified after copper supplementation. However, CCS and SOD1 mRNA levels were reduced in PMNCs after copper supplementation in the individuals with the high Cp values, suggesting that they should be further explored as biomarkers of moderate copper overload in humans.en_US
Patrocinadordc.description.sponsorshipThis investigation was funded in part by the International Copper Association (ICA, New York); by grant Fondecyt 1030618 and by CINUT, Chile (Corporación de Ayuda a la Investigación en Nutrición), in the form of unrestricted research grants. MS was a recipient of a CONICYT Fellowship.en_US
Publisherdc.publisherELSEVIER SCIENCE INCen_US
Keywordsdc.subjectCCS; SOD1en_US
Títulodc.titleCCS and SOD1 mRNA are reduced after copper supplementation in peripheral mononuclear cells of individuals with high serum ceruloplasmin concentrationen_US
Document typedc.typeArtículo de revistaen_US

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