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Equol and daidzein decrease migration, invasion and matrix metalloproteinase (MMPs) gene expression in prostate cancer cell lines, DU-145 and PC-3

Authordc.contributor.authorLeiva, Bárbara 
Authordc.contributor.authorCarrasco, Ivo 
Authordc.contributor.authorMontenegro, Iván 
Authordc.contributor.authorGaete, Leonardo 
Authordc.contributor.authorLemus Gutiérrez, Igor 
Authordc.contributor.authorTchernitchin, Andrei 
Authordc.contributor.authorBustamante, Rodrigo 
Authordc.contributor.authorPárraga, Mario 
Authordc.contributor.authorVillena, Joan 
Admission datedc.date.accessioned2015-09-15T19:47:35Z
Available datedc.date.available2015-09-15T19:47:35Z
Publication datedc.date.issued2015
Cita de ítemdc.identifier.citationBoletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 14 (3): 251 - 262, 2015en_US
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/133675
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractThis search is focused on the study of diet compounds that may have any potential chemopreventive effect against cancer. Some compounds that fulfill this requirement are phytoestrogens. Among them we find genistein (1), the most studied, daidzein (2) and equol (3) (figure 1). To compare the sensitivities of different prostate cancer cells to phytoestrogen treatment, sulphorhodamine B dye assay was performed to determine cell viability. DU-145 and PC-3 prostate cancer cell lines treated with various doses of phytoestrogen (0-12.5-25-50 and 100 mu M) for different times (24, 48 and 72h). For cell invasion or migration assay cells were seeded in a Transwell chamber with or without coating Matrigel respectively. DU-145 and PC-3 cells were treated previously with phytoestrogen (50 mu M) for 24h. The study showed that equol, daidzein and genistein inhibited migration and invasion in prostate cancer cell lines. Moreover, we analyzed the effects of phytoestrogens in MMP-2 and MMP-9 mRNA expression by RT-PCR. The results indicated that equol, daidzein and genistein diminished the expression of MMP-2 and MMP-9 in a cell-dependent manner. Our data suggested that equol, daidzein and genistein inhibited migration and invasion in prostate cancer cell lines. Moreover, the results also suggest that down-regulation of MMP-2 and MMP-9 might be involved in the inhibition of invasion of PC-3 and DU-145 cells after genistein, daidzein and equol treatment.en_US
Patrocinadordc.description.sponsorshipDIPUV of the Universidad de Valparaiso 27/2006 Research Team Grant in Science and Technology, Bicentennial Program in Science and Technology, CONICYT, Chile ACT07en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherUniversidad de Santiago de Chileen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectEquolen_US
Keywordsdc.subjectDaidzeinen_US
Keywordsdc.subjectMigrationen_US
Keywordsdc.subjectInvasionen_US
Keywordsdc.subjectMMPen_US
Keywordsdc.subjectProstate canceren_US
Títulodc.titleEquol and daidzein decrease migration, invasion and matrix metalloproteinase (MMPs) gene expression in prostate cancer cell lines, DU-145 and PC-3en_US
Document typedc.typeArtículo de revistaen_US


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile