Flavonoids in Microheterogeneous Media, Relationship between Their Relative Location and Their Reactivity towards Singlet Oxygen
Author
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Günther Sapunar, Germán
Author
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Berríos, Eduardo
Author
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Pizarro, Nancy
Author
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Valdés Camus, Karina
Author
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Montero, Guillermo
Author
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Arriagada, Francisco
Author
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Morales Montecinos, Javier
Admission date
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2015-09-29T18:49:27Z
Available date
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2015-09-29T18:49:27Z
Publication date
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2015
Cita de ítem
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PLoS One Volumen: 10 Número: 6 (Jun 2015)
en_US
Identifier
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DOI: 10.1371/journal.pone.0129749
Identifier
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https://repositorio.uchile.cl/handle/2250/133961
General note
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Artículo de publicación ISI
en_US
Abstract
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In this work, the relationship between the molecular structure of three flavonoids (kaempferol, quercetin and morin), their relative location in microheterogeneous media (liposomes and erythrocyte membranes) and their reactivity against singlet oxygen was studied. The changes observed in membrane fluidity induced by the presence of these flavonoids and the influence of their lipophilicity/hydrophilicity on the antioxidant activity in lipid membranes were evaluated by means of fluorescent probes such as Laurdan and diphenylhexatriene (DPH). The small differences observed for the value of generalized polarization of Laurdan (GP) curves in function of the concentration of flavonoids, indicate that these three compounds promote similar alterations in liposomes and erythrocyte membranes. In addition, these compounds do not produce changes in fluorescence anisotropy of DPH, discarding their location in deeper regions of the lipid bilayer. The determined chemical reactivity sequence is similar in all the studied media (kaempferol < quercetin < morin). Morin is approximately 10 times more reactive than quercetin and 20 to 30 times greater than kaempferol, depending on the medium.