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Real evidence about zeolite as microorganisms immobilizer in anaerobic fluidized bed reactors

Authordc.contributor.authorFernández, N. 
Authordc.contributor.authorMontalvo, S. 
Authordc.contributor.authorFernández Polanco, F. 
Authordc.contributor.authorGuerrero, L. 
Authordc.contributor.authorCortés, I. 
Authordc.contributor.authorBorja, R. 
Authordc.contributor.authorSánchez, E. 
Authordc.contributor.authorTravieso, L. 
Cita de ítemdc.identifier.citationProcess Biochemistry 42 (2007) 721–728en_US
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractUsing the scanning electronic microscopy, it was observed that natural zeolite possesses excellent physical characteristics as a support medium in anaerobic fluidized bed reactors (AFBR). Samples for biomass analysis were taken from two identical laboratory-scale AFBR (R-1 and R-2), which were operated with 25% of fluidization. These reactors treated distillery wastewaters (vinasses) at mesophilic temperature (30 2 8C). The experiments were carried out with 0.25–0.50 and 0.50–0.80 mm zeolite particle diameter in reactors R-1 and R-2, respectively. The biomass concentration attached to zeolite in both reactors was found to be in the range of 40–45 g volatile solids/l. COD removal efficiencies as high as 90% were achieved at organic loading rate (OLRs) of up to 20 g COD/l day. The volatile fatty acid (VFA) levels were always lower that the suggested limits for digester failure. The yield coefficient of methane production was 0.29 l CH4(at STP)/g COD consumed and was virtually independent of the OLR applied. A hybridization technique (fluorescence in situ hybridization, FISH) helped determine the predominant anaerobic microorganisms that colonized the natural zeolite, which were found to be Methanosaeta and Methanosarcinaceae, observing a reduced number of sulphate reducing bacteria. The results obtained for reactors R-1 and R-2 were very similar, showing that the particle size did not significantly influence the microbial community immobilized on zeolite.en_US
Patrocinadordc.description.sponsorshipFONDECYT Project No. 1020201, Chile, and to the "Ministerio de Educación y Ciencia" and "Junta de Anadalucía" of Spainen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectAnaerobic fluidized bed reactors (AFBR)en_US
Keywordsdc.subjectScanning electronic microscopy (SEM)en_US
Keywordsdc.subjectMicrobial communitiesen_US
Keywordsdc.subjectFluorescence in situ hybridization (FISH) techniqueen_US
Títulodc.titleReal evidence about zeolite as microorganisms immobilizer in anaerobic fluidized bed reactorsen_US
Document typedc.typeArtículo de revistaen_US

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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile