Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
Author
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Narváez, Cristóbal
Author
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Ríos, Juan Manuel
Author
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Píriz, Gabriela
Author
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Sánchez Hernández, Juan C.
Author
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Sabat Kirkwood, Alejandro Pablo
Admission date
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2016-05-19T21:08:26Z
Available date
dc.date.available
2016-05-19T21:08:26Z
Publication date
dc.date.issued
2016
Cita de ítem
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Chemosphere 144 (2016) 775–784
en_US
Identifier
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DOI: 10.1016/j.chemosphere.2015.09.060
Identifier
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https://repositorio.uchile.cl/handle/2250/138393
General note
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Artículo de publicación ISI
en_US
Abstract
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Effects of pesticides on non-target organisms have been studied in several taxa at different levels of biological organization, from enzymatic to behavioral responses. Although the physiological responses may be associated with higher energy costs, little is known about metabolic costs of pesticide detoxification in birds. To fill this gap, we exposed orally (diet) 15-d old Coturnix cotumix japonica individuals to sublethal doses of chlorpyrifos (10 and 20 mg active ingredient/kg dry food) for four weeks. Carboxylesterase (CbE), butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities were periodically measured in multiple tissues along with measurements of resting (RMR) and maximum metabolic rates (M-sum). Furthermore, glucuronic acid in bird excreta was also assessed at the end of the trial. While CbE and BChE activities were inhibited by chlorpyrifos in all tissues during the third and fourth weeks following pesticide treatment, AChE activity was unaffected. At this sampling times, both M-sum and RMR expansibility decreased. These results suggest that the exposure to chlorpyrifos caused a negative effect on aerobic performance. Additionally, excretion rate of glucuronic acid was up to 2-fold higher in the 20-mg/kg group than in the control and 10-mg/kg chlorpyrifos groups. The inhibition of CbE and BChE activities corroborated that these enzymes are fulfilling their role as bioscavengers for organophosphate pesticides, decreasing its concentration and thus protecting AChE activity against inhibition by chlorpyrifos.
en_US
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica
22111010
Fondo Nacional de Desarrollo Cientifico y Tecnologico (Chile)
1120276