High throughput selection of novel plant growth regulators: Assessingthe translatability of small bioactive molecules from Arabidopsis tocrops
Author
dc.contributor.author
Rodriguez Furlán, Cecilia
Author
dc.contributor.author
Miranda Flores, Giovanna
Author
dc.contributor.author
Reggiardo, Martín
Author
dc.contributor.author
Hicks, Glenn
Author
dc.contributor.author
Norambuena Morales, Lorena
Admission date
dc.date.accessioned
2016-06-29T22:06:35Z
Available date
dc.date.available
2016-06-29T22:06:35Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
Plant Science 245 (2016) 50–60
en_US
Identifier
dc.identifier.issn
0168-9452
Identifier
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DOI: 10.1016/j.plantsci.2016.01.001
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/139291
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
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tPlant growth regulators (PGRs) have become an integral part of agricultural and horticultural practices.Accordingly, there is an increased demand for new and cost-effective products. Nevertheless, the marketis limited by insufficient innovation. In this context chemical genomics has gained increasing attentionas a powerful approach addressing specific traits. Here is described the successful implementation of ahighly specific, sensitive and efficient high throughput screening approach using Arabidopsis as a model.Using a combination of techniques, 10,000 diverse compounds were screened and evaluated for severalimportant plant growth traits including root and leaf growth. The phenotype-based selection allowedthe compilation of a collection of putative Arabidopsis growth regulators with a broad range of activitiesand specificities. A subset was selected for evaluating their bioactivity in agronomically valuable plants.Their validation as growth regulators in commercial species such as tomato, lettuce, carrot, maize andturfgrasses reinforced the success of the screening in Arabidopsis and indicated that small moleculesactivity can be efficiently translated to commercial species. Therefore, the chemical genomics approachin Arabidopsis is a promising field that can be incorporated in PGR discovery programs and has a greatpotential to develop new products that can be efficiently used in crops.
en_US
Patrocinador
dc.description.sponsorship
FONDEF
CA12I10206
FONDECYT
1120289
US Department of Energy
DE-FG02-02ER15295