Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii
Author
dc.contributor.author
Gimpel, Javier
Author
dc.contributor.author
Nour-Eldin, Hussam
Author
dc.contributor.author
Scranton, Melissa
Author
dc.contributor.author
Li, Daphne
Author
dc.contributor.author
Mayfield, Stephen
Admission date
dc.date.accessioned
2016-12-19T15:34:58Z
Available date
dc.date.available
2016-12-19T15:34:58Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
ACS Synth. Biol. 2016, 5, 589−596
es_ES
Identifier
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10.1021/acssynbio.5b00076
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/141958
Abstract
dc.description.abstract
Oxygenic photosynthesis provides the energy to produce all food and most of the fuel on this planet. Photosystem II (PSII) is an essential and rate-limiting component of this process. Understanding and modifying PSII function could provide an opportunity for optimizing photosynthetic biomass production, particularly under specific environmental conditions. PSII is a complex multisubunit enzyme with strong interdependence among its components. In this work, we have deleted the six core genes of PSIl in the eukaryotic alga Chlamydomonas reinhardtii and refactored them in a single DNA construct. Complementation of the knockout strain with the core PSII synthetic module from three different green algae resulted in reconstitution of photosynthetic activity to 85, 55, and 53% of that of the wild-type, demonstrating that the PSII core can be exchanged between algae species and retain function. The strains, synthetic cassettes, and refactoring strategy developed for this study demonstrate the potential of synthetic biology approaches for tailoring oxygenic photosynthesis and provide a powerful tool for unraveling PSII structure-function relationships.
es_ES
Patrocinador
dc.description.sponsorship
U.S. Department of Energy
DE-EE-0003373
California Energy Commission Initiative for Large Molecule Sustainable Fuels
500-10-039
Danish Research Council for Technology and Production
09-065827/274-08-0354