Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat
Author
dc.contributor.author
Pealoza, E.
Author
dc.contributor.author
Santiago, M.
Author
dc.contributor.author
Cabrera, S.
Author
dc.contributor.author
Muñoz, G.
Author
dc.contributor.author
Corcuera, L. J.
Author
dc.contributor.author
Silva Ascencio, Herman
Admission date
dc.date.accessioned
2018-05-25T15:01:46Z
Available date
dc.date.available
2018-05-25T15:01:46Z
Publication date
dc.date.issued
2017
Cita de ítem
dc.identifier.citation
Biologia Plantarum 61 (3): 453-462, 2017
es_ES
Identifier
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10.1007/s10535-016-0672-9
Identifier
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https://repositorio.uchile.cl/handle/2250/148132
Abstract
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The root specificity and phosphate (Pi) deficiency responsiveness of high-affinity phosphate transporter (PHT1) genes point to their promoters as a sustainable system to drive Pi acquisition-related transgenes in plants. In this study, a 3-kb promoter of the AtPHT1;4 gene from Arabidopsis thaliana fused to the beta-glucuronidase (GUS) reporter gene was biolistically introduced into wheat (Triticum aestivum L.) and functionally characterized in transgenic plants grown in hydroponics and in pots with soil under various Pi supply rates. From among 27 T-1 progeny derived from 250 T-0, four transgenic lines reached T-3, with two of them showing detectable GUS activity in the roots of T-4 plants. An unusually high number of transgene insertions characterized these transgenic lines, along with an irregular pattern of histochemical GUS staining and weak GUS activity. GUS expression driven by AtPHT1;4 was consistently higher under most assay conditions, as it was unaffected by 0 to 0.5 mM Pi in hydroponically grown plants, as well as by 16 to 20 mg(P) kg(-1)(soil) in potted plants. Raising the soil P up to or above 40 mg kg(-1) significantly down-regulated the quantity of GUS transcripts. These results show that the responsiveness of the AtPHT1;4 promoter to Pi availability in transgenic wheat was restricted to soil-grown plants, which highlighted the relevance of the substrate and Pi supply rates in assessing molecular responses to Pi deficiency.