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A CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+ -activated Cl- current when transfected into HEK293

Authordc.contributor.authorMura, Casilda V. 
Authordc.contributor.authorDelgado Arriagada, Ricardo 
Authordc.contributor.authorDelgado, María Graciela 
Authordc.contributor.authorRestrepo, Diego 
Authordc.contributor.authorBacigalupo Vicuña, Juan 
Cita de ítemdc.identifier.citationBMC Neuroscience (2017) 18: 61es_ES
Abstractdc.description.abstractBackground: CLCA is a family of metalloproteases that regulate Ca2+-activated Cl- fluxes in epithelial tissues. In HEK293 cells, CLCA1 promotes membrane expression of an endogenous Anoctamin 1 (ANO1, also termed TMEM16A)-dependent Ca2+-activated Cl- current. Motif architecture similarity with CLCA2, 3 and 4 suggested that they have similar functions. We previously detected the isoform CLCA4L in rat olfactory sensory neurons, where Anoctamin 2 is the principal chemotransduction Ca2+-activated Cl-channel. We explored the possibility that this protein plays a role in odor transduction. Results: We cloned and expressed CLCA4L from rat olfactory epithelium in HEK293 cells. In the transfected HEK293 cells we measured a Cl-selective Ca2+-activated current, blocked by niflumic acid, not present in the non-transfected cells. Thus, CLCA4L mimics the CLCA1 current on its ability to induce the ANO1-dependent Ca2+-activated Cl-current endogenous to these cells. By immunocytochemistry, a CLCA protein, presumably CLCA4L, was detected in the cilia of olfactory sensory neurons co-expressing with ANO2. Conclusion: These findings suggests that a CLCA isoform, namely CLCA4L, expressed in OSN cilia, might have a regulatory function over the ANO2-dependent Ca2+-activated Cl-channel involved in odor transduction.es_ES
Patrocinadordc.description.sponsorshipFONDECYT 1100682 1140520 NIH DC014253es_ES
Publisherdc.publisherBIOMED Central Ltd.es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceBMC Neurosciencees_ES
Keywordsdc.subjectCa2+ -activated Cl- currentes_ES
Keywordsdc.subjectAnoctamin channelses_ES
Keywordsdc.subjectOlfactory ciliaes_ES
Títulodc.titleA CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+ -activated Cl- current when transfected into HEK293es_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile