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Antifungal activity of the essential oil obtained from Cryptocarya alba against infection in honey bees by Nosema ceranae

Authordc.contributor.authorBravo, J. 
Authordc.contributor.authorCarbonell, V. 
Authordc.contributor.authorSepúlveda, B. 
Authordc.contributor.authorDelporte Vergara, Carla 
Authordc.contributor.authorValdovinos, C. E. 
Authordc.contributor.authorMartín Hernández, R. 
Authordc.contributor.authorHiges, M. 
Cita de ítemdc.identifier.citationJournal of Invertebrate Pathology, 149 (2017): 141–147es_ES
Abstractdc.description.abstractThe honeybee disease nosemosis type C is a serious problem since its causative agent, microsporidium Nosema ceranae, is widespread among adult honey bees. Some of the feasible alternative treatments that are used to control this disease are plant extracts. The aim of the present work was to evaluate the effects of essential oils of Chilean plant species, such as Cryptocarya alba, which is used against N. ceranae, and to identify and quantify the majority active compounds in the EO as well as their potential use for the control of nosemosis. Essential oils were obtained using the stripping steam technique with Clevenger equipment and were subsequently analyzed by Gas chromatography mass spectrometry. Mortality was recorded daily over at least 8 days as worker honeybees were exposed to a range of doses of EO dispersed in a sucrose solution. C. alba oil appears to be nontoxic to A. mellifera adults at the tested concentration (the same concentration inhibits the growth of N. coronae), showing that this oil can be used for the treatment of nosemosis. EO effectiveness was demonstrated against N. ceranae by calculating the percentage of decrease in infected bees from untreated infected groups vs infected groups treated with EO or the reference drug fumagillin. It was determined that a dose of 4 EO/bee was most effective in controlling N. coronae development. We determined innocuous doses of C. alba essential oil for honeybees. We demonstrated the antifungal activity of C. alba EO at 4 mu g/bee against N. coronae and compared it to its major monoterpenes, such as beta-phellandrene (20 mu g/bee), eucalyptol (20 mu g/bee) and a-terpineol (20 lig/bee). The major compounds of C. alba EO, alpha-terpineol, eucalyptol and beta-phellandrene, had significant effects against Apis mellifera infection by N. coronae, but the antifungal effect of the complete essential oil on N. ceranae was larger than the effect of alpha-terpineol, eucalyptol or beta-phellandrene separately, showing that C. alba oil may be a candidate for the treatment or prevention of nosemosis.es_ES
Patrocinadordc.description.sponsorshipScholarship to Doctoral Thesis CONICYT Scholarship Mecesup Internship Spanish Ministry "Economia y Competitividad" INIA-FEDER 2012-00072CO2-01es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceJournal of Invertebrate Pathologyes_ES
Keywordsdc.subjectApis melliferaes_ES
Keywordsdc.subjectEssential oiles_ES
Keywordsdc.subjectNosema ceranaees_ES
Keywordsdc.subjectAlpha Terpineoles_ES
Keywordsdc.subjectBeta Phellandrenees_ES
Títulodc.titleAntifungal activity of the essential oil obtained from Cryptocarya alba against infection in honey bees by Nosema ceranaees_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile