An HPLC method for the determination of isoflavones and the evaluation of their antioxidant capacity in both homogeneous and microheterogeneous systems
Author
dc.contributor.author
Montero, Guillermo
Author
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Günther Sapunar, Germán
Author
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Valdés Camus, Karina
Author
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Arriagada, Francisco
Author
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Morales, Javier O.
Admission date
dc.date.accessioned
2018-07-30T20:11:11Z
Available date
dc.date.available
2018-07-30T20:11:11Z
Publication date
dc.date.issued
2018
Cita de ítem
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Journal of AOAC International Volumen: 101 Número: 1 Páginas: 235-241
es_ES
Identifier
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10.5740/jaoacint.17-0104
Identifier
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https://repositorio.uchile.cl/handle/2250/150457
Abstract
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In this work, we developed an HPLC method to simultaneously quantify and hence evaluate the stability, distribution, and antioxidant capacity of six isoflavones: genistein, genistin, daidzein, daidzin, glycitin, and biochanin A. Isoflavones have been described as having an important estrogenic activity to treat menopausal symptoms and can reduce postmenopausal bone loss and also participate in the prevention of cardiovascular diseases. These beneficial properties are believed derived from their capacity to act as free-radical scavengers. Isoflavones are formulated in capsules and creams and also can be used as antioxidants in liposomes. HPLC separation was achieved on an Agilent Hypersil ODS C18 column. The mobile phase consisted of 0.02-0.2% orthophosphoric acid in water acetonitrile with gradient elution. The diode array detector was operated at 260 nm. The hydrophobicity of isoflavones was determined through their distribution in octanol buffer. These results allowed us to establish a relation between chemical structure, pKa, lipophilicity, and the characteristics of the dispersion medium. Photolysis of hydrogen peroxide was used to measure the HO center dot scavenging capability of isoflavones. In liposomes, the order of reactivity of the studied compounds was genistein > biochanin A > genistin > daidzein > daidzin > glycitin.
es_ES
Patrocinador
dc.description.sponsorship
Fondo Nacional de Desarrollo Cientifico y Tecnológico
1160757
An HPLC method for the determination of isoflavones and the evaluation of their antioxidant capacity in both homogeneous and microheterogeneous systems