Matrix metalloproteinase 9 displays a particular time response to acute stress: variation in its levels and activity distribution in rat hippocampus
Author
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Aguayo, Felipe I.
Author
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Pacheco Zapata, Aníbal
Author
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García Rojo, Gonzalo
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Pizarro Bauerle, Javier
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Doberti Martínez, Ana Valentina
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Tejos Bravo, Macarena
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García-Pérez, María A.
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Rojas, Paulina S.
Author
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Fiedler Temer, Jenny
Admission date
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2018-09-27T19:22:29Z
Available date
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2018-09-27T19:22:29Z
Publication date
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2018-05
Cita de ítem
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ACS Chem. Neurosci. 2018, 9, 945−956
es_ES
Identifier
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10.1021/acschemneuro.7b00387
Identifier
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https://repositorio.uchile.cl/handle/2250/151817
Abstract
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A single stress exposure facilitates memory formation through neuroplastic processes that reshape excitatory synapses in the hippocampus, probably requiring changes in extracellular matrix components. We tested the hypothesis that matrix metalloproteinase 9 (MMP-9), an enzyme that degrades components of extracellular matrix and synaptic proteins such as beta-dystroglycan (beta-DG(43)), changes their activity and distribution in rat hippocampus during the acute stress response. After 2.5 h of restraint stress, we found (i) increased MMP-9 levels and potential activity in whole hippocampal extracts, accompanied by beta-DG(43) cleavage, and (ii) a significant enhancement of MMP-9 immunoreactivity in dendritic fields such as stratum radiatum and the molecular layer of hippocampus. After 24 h of stress, we found that (i) MMP-9 net activity rises at somatic field, i.e., stratum pyramidale and granule cell layers, and also at synaptic field, mainly stratum radiatum and the molecular layer of hippocampus, and (ii) hippocampal synaptoneurosome fractions are enriched with MMP-9, without variation of its potential enzymatic activity, in accordance with the constant level of cleaved beta-DG(43). These findings indicate that stress triggers a peculiar timing response in the MMP-9 levels, net activity, and subcellular distribution in the hippocampus, suggesting its involvement in the processing of substrates during the stress response.