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Induction of amphibian oocyte maturation by polyvalent cations and alkaline pH in the absence of potassium ions

Authordc.contributor.authorKofoid, Eric C. 
Authordc.contributor.authorKnauber, Donna C. 
Authordc.contributor.authorAllende, Jorge E. 
Admission datedc.date.accessioned2018-12-20T14:08:12Z
Available datedc.date.available2018-12-20T14:08:12Z
Publication datedc.date.issued1979
Cita de ítemdc.identifier.citationDevelopmental Biology, Volumen 72, Issue 2, 2018, Pages 374-380
Identifierdc.identifier.issn00121606
Identifierdc.identifier.other10.1016/0012-1606(79)90126-X
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/154133
Abstractdc.description.abstractThe maturation of Xenopus laevis oocytes was studied in media free of added potassium salts. Under these conditions maturation could be triggered by 1 mM Mn2+ and La3+ and, to a lesser extent, by 2-4 mM Ca2+ and Mg2+. Maturation induced by 1.5 mM Mn2+ was inhibited by K+ concentrations above 0.25 mM. Potassium was inhibitory when added up to 2 hr before germinal vesicle breakdown occurred. In potassium-free media, maturation could be induced by incubation of oocytes under mild alkaline media (pH 8.5-9). A high percentage of medium-sized oocytes (stage IV according to Dumont) was induced to mature by progesterone in the absence of potassium. Maturation of oocytes in potassium-free media was normal by the criteria of germinal vesicle breakdown, production of maturation promoting factor, vitelline membrane activation, and inhibition by known maturation inhibitors. © 1979.
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceDevelopmental Biology
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectDevelopmental Biology
Keywordsdc.subjectCell Biology
Títulodc.titleInduction of amphibian oocyte maturation by polyvalent cations and alkaline pH in the absence of potassium ions
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile