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Induction of amphibian oocyte maturation by polyvalent cations and alkaline pH in the absence of potassium ions

Authordc.contributor.authorKofoid, Eric C. 
Authordc.contributor.authorKnauber, Donna C. 
Authordc.contributor.authorAllende, Jorge E. 
Cita de ítemdc.identifier.citationDevelopmental Biology, Volumen 72, Issue 2, 2018, Pages 374-380
Abstractdc.description.abstractThe maturation of Xenopus laevis oocytes was studied in media free of added potassium salts. Under these conditions maturation could be triggered by 1 mM Mn2+ and La3+ and, to a lesser extent, by 2-4 mM Ca2+ and Mg2+. Maturation induced by 1.5 mM Mn2+ was inhibited by K+ concentrations above 0.25 mM. Potassium was inhibitory when added up to 2 hr before germinal vesicle breakdown occurred. In potassium-free media, maturation could be induced by incubation of oocytes under mild alkaline media (pH 8.5-9). A high percentage of medium-sized oocytes (stage IV according to Dumont) was induced to mature by progesterone in the absence of potassium. Maturation of oocytes in potassium-free media was normal by the criteria of germinal vesicle breakdown, production of maturation promoting factor, vitelline membrane activation, and inhibition by known maturation inhibitors. © 1979.
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceDevelopmental Biology
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectDevelopmental Biology
Keywordsdc.subjectCell Biology
Títulodc.titleInduction of amphibian oocyte maturation by polyvalent cations and alkaline pH in the absence of potassium ions
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile