Show simple item record

Phosphorylation of phosphatidylinositol by transverse tubule vesicles and its possible role in excitation-contraction coupling

Authordc.contributor.authorHidalgo Tapia, María Cecilia 
Authordc.contributor.authorCarrasco, María Angélica 
Authordc.contributor.authorMagendzo, Karin 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Admission datedc.date.accessioned2018-12-20T14:08:20Z
Available datedc.date.available2018-12-20T14:08:20Z
Publication datedc.date.issued1986
Cita de ítemdc.identifier.citationFEBS Letters, Volumen 202, Issue 1, 2018, Pages 69-73
Identifierdc.identifier.issn00145793
Identifierdc.identifier.other10.1016/0014-5793(86)80651-2
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/154186
Abstractdc.description.abstractPhosphorylation of phosphatidylinositol to phosphatidylinositol 4-monophosphate and to phosphatidyl-inositol 4,5-bisphosphate was demonstrated in transverse-tubule membranes isolated from frog skeletal muscle using [γ-32P]ATP as substrate. At millimolar concentrations of Mg2+ both phosphorylation reactions were completed within 15 s at 25°C. Isolated sarcoplasmic reticulum vesicles phosphorylated phosphatidyl-inositol to phosphatidylinositol 4-phosphate with a lower specific activity than the transverse tubules, and lacked the ability to produce phosphatidylinositol 4,5-bisphosphate. These findings show, for the first time, that isolated transverse-tubule membranes carry out one of the steps required to sustain a role for inositol trisphosphate as the physiological messenger in excitation-contraction coupling in skeletal muscle. The finding that 0.5 mM tetracaine apparently inhibits the phosphorylation of phosphatidylinositol 4-phosphate to phosphatidylinositol 4,5-bisphosphate also su
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceFEBS Letters
Keywordsdc.subjectBiophysics
Keywordsdc.subjectStructural Biology
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMedicine (all)
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectGenetics
Keywordsdc.subjectCell Biology
Títulodc.titlePhosphorylation of phosphatidylinositol by transverse tubule vesicles and its possible role in excitation-contraction coupling
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile