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Iron-induced oxidative damage in colon carcinoma (Caco-2) cells

Authordc.contributor.authorNúñez González, Marco 
Authordc.contributor.authorTapia, Victoria 
Authordc.contributor.authorToyokuni, Shinya 
Authordc.contributor.authorOkada, Shigeru 
Admission datedc.date.accessioned2018-12-20T15:20:32Z
Available datedc.date.available2018-12-20T15:20:32Z
Publication datedc.date.issued2001
Cita de ítemdc.identifier.citationFree Radical Research, Volumen 34, Issue 1, 2018, Pages 57-68
Identifierdc.identifier.issn10715762
Identifierdc.identifier.other10.1080/10715760100300061
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/158812
Abstractdc.description.abstractIntestinal epithelial cells have an active apical iron uptake system that is involved in the regulated absorption of iron. By the action of this system, intestinal cells acquire increasing amounts of iron with time. Since intracellular reactive iron is a source of free radicals and a possible cause of colon carcinoma, this study analyzed the oxidative damages generated by iron accumulation in Caco-2 cells. Cells cultured with increasing concentrations of iron increased both total intracellular iron and the reactive iron pool, despite an active IRE/IRP system, which regulates intracellular iron levels. Increasing concentrations of iron resulted in increased protein oxidative damage, as shown by the immunoreactivity for 4-hydroxy-2-nonenal-modified proteins, and markedly induced DNA oxidation determined by 8-hydroxy-2′-deoxyguanidine production. Iron also impaired cell viability, resulting in increased cell death after 6 days of culture. In summary, iron accumulation by intestinal Caco-2
Lenguagedc.language.isoen
Publisherdc.publisherHarwood Academic Publishers GmbH
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceFree Radical Research
Keywordsdc.subject8-hydroxy-guanidine
Keywordsdc.subjectCell death
Keywordsdc.subjectFerritin
Keywordsdc.subjectHNE
Keywordsdc.subjectIRP
Keywordsdc.subjectOxidative damage
Títulodc.titleIron-induced oxidative damage in colon carcinoma (Caco-2) cells
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile