The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)
Author
dc.contributor.author
Beltrán Lissabet, Jorge Félix
Author
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Herrera Belén, Lisandra
Author
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LeeEstevez, Manuel
Author
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Risopatrón, Jennie
Author
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Valdebenito, Iván
Author
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Figueroa, Elías
Author
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Farías, Jorge G.
Admission date
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2020-05-11T22:20:28Z
Available date
dc.date.available
2020-05-11T22:20:28Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Comparative Biochemistry and Physiology, Part A 241 (2020) 110634
es_ES
Identifier
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10.1016/j.cbpa.2019.110634
Identifier
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https://repositorio.uchile.cl/handle/2250/174661
Abstract
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Among all the Ca2+ channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process. In fish sperm, the calcium channel plays a key role in sperm activation. However, the functionality of the CatSper channels has not been studied in any of the fish species. For the first time, we studied the relationship of the CatSper channel with sperm motility in a fish, using Atlantic salmon (Salmo salar) as the model. The results of our study showed that the CatSper channel in Salmo salar has chemical-physical characteristics similar to those reported for mammalian CatSper channels. In this work, it was shown that Salmo salar CatSper 3 protein has a molecular weight of approximately 55-kDa similar to Homo sapiens CatSper 3. In silico analyses suggest that this channel forms a heterotetramer sensitive to the specific inhibitor HC-056456, with a binding site in the center of the pore of the CatSper channel, hindering or preventing the influx of Ca (2+) ions. The in vitro assay of the sperm motility inhibition of Salmo salar with the inhibitor HC-056456 showed that sperm treated with this inhibitor significantly reduced the total and progressive motility (p < .0001), demonstrating the importance of this ionic channel for this cell. The complementation of the in silico and in vitro analyses of the present work demonstrates that the CatSper channel plays a key role in the regulation of sperm motility in Atlantic salmon.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT), CONICYT FONDECYT: 1180387.
UFRO Scholarship.
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT), CONICYT FONDECYT: 3180765.
DIUFRO: DIE14-0001, DI12-PEO1.