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Authordc.contributor.authorD'Espessailles Tapia, Amanda 
Authordc.contributor.authorSantillana, Natalia 
Authordc.contributor.authorSanhueza, Sofía 
Authordc.contributor.authorFuentes, Cecilia 
Authordc.contributor.authorCifuentes Koster, Mariana 
Cita de ítemdc.identifier.citationMolecular and Cellular Endocrinology 501 (2020) 110654es_ES
Abstractdc.description.abstractExcess adipose tissue (AT) associates with inflammation and obesity-related diseases. We studied whether calcium-sensing receptor (CaSR)-mediated NLRP3 inflammasome activation in THP-1 macrophages elevates inflammation in LS14 preadipocytes, modeling deleterious AT cell crosstalk. THP-1 macrophages exposed to cinacalcet (CaSR activator, 2 mu M, 4 h) showed elevated proinflammatory marker and NLRP3 inflammasome mRNA, pro-IL-1 beta protein and caspase-1 activity, whereas preincubation with CaSR negative modulators prevented these effects. The key NLRP3 inflammasome component ASC was silenced (siRNA) in THP-1 cells, and inflammasome activation was evaluated (qPCR, Western blot, caspase-1 activity) or they were further cultured to obtain conditioned medium (CoM). Exposure of LS14 preadipocytes to CoM from cinacalcet-treated THP-1 elevated LS14 proinflammatory cytokine expression, which was abrogated by THP-1 inflammasome silencing. Thus, CaSR activation elevates THP-1-induced inflammation in LS14 preadipocytes, via macrophage NLRP3 inflammasome activation. Modulating CaSR activation may prevent deleterious proinflammatory cell crosstalk in AT, a promising approach in obesity-related metabolic disorders.es_ES
Patrocinadordc.description.sponsorshipComisión Nacional de Investigación Científica y Tecnológica (CONICYT), CONICYT FONDECYT: 1150651. Comisión Nacional de Investigación Científica y Tecnológica (CONICYT): 21140284, 22170739.es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceMolecular and Cellular Endocrinologyes_ES
Keywordsdc.subjectCalcium sensing receptores_ES
Keywordsdc.subjectNLRP3 inflammasomees_ES
Títulodc.titleCalcium sensing receptor activation in THP-1 macrophages triggers NLRP3 inflammasome and human preadipose cell inflammationes_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile