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Authordc.contributor.authorRobles Planells, Claudia 
Authordc.contributor.authorSánchez-Guerrero, Giselle 
Authordc.contributor.authorBarrera Ávalos, Carlos 
Authordc.contributor.authorMatiacevich, Silvia 
Authordc.contributor.authorRojo, Leonel E. 
Authordc.contributor.authorPavéz, Jorge 
Authordc.contributor.authorSalas Huenuleo, Edison 
Authordc.contributor.authorKogan, Marcelo J. 
Authordc.contributor.authorEscobar Álvarez, Alejandro 
Authordc.contributor.authorMilla, Luis A. 
Authordc.contributor.authorFernández, Ricardo 
Authordc.contributor.authorImarai, Mónica 
Authordc.contributor.authorSpencer, Eugenio 
Authordc.contributor.authorHuidobro Toro, Juan Pablo 
Authordc.contributor.authorAcuña Castillo, Claudio 
Admission datedc.date.accessioned2020-06-10T19:29:57Z
Available datedc.date.available2020-06-10T19:29:57Z
Publication datedc.date.issued2020
Cita de ítemdc.identifier.citationMediators Inflamm. (2020): 8680692es_ES
Identifierdc.identifier.other10.1155/2020/8680692
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/175385
Abstractdc.description.abstractOncolytic virus therapy has been tested against cancer in preclinical models and clinical assays. Current evidence shows that viruses induce cytopathic effects associated with fusogenic protein-mediated syncytium formation and immunogenic cell death of eukaryotic cells. We have previously demonstrated that tumor cell bodies generated from cells expressing the fusogenic protein of the infectious salmon anemia virus (ISAV-F) enhance crosspriming and display prophylactic antitumor activity against melanoma tumors. In this work, we evaluated the effects of the expression of ISAV-F on the B16 melanoma model, both in vitro and in vivo, using chitosan nanoparticles as transfection vehicle. We confirmed that the transfection of B16 tumor cells with chitosan nanoparticles (NP-ISAV) allows the expression of a fusogenically active ISAV-F protein and decreases cell viability because of syncytium formation in vitro. However, the in vivo transfection induces a delay in tumor growth, without inducing changes on the lymphoid populations in the tumor and the spleen. Altogether, our observations show that expression of ISAV fusion protein using chitosan nanoparticles induces cell fusion in melanoma cells and slight antitumor response.es_ES
Patrocinadordc.description.sponsorshipComisión Nacional de Investigación Cientifica y Tecnológica (CONICYT) CONICYT FONDECYT 1161015 11140731 11140915 1180666 Programa de Equipamiento Científico y Tecnológico EQM150069 EQM190024 Direccion de Investigacion Científica y Tecnologica 021943AC 021801LR 021801MB Comisión Nacional de Investigación Científica y Tecnológica 21171377 Fondap 15130011es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherHindawies_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceMediators of Inflammationes_ES
Keywordsdc.subjectFusogenic membrane-glycoproteinses_ES
Keywordsdc.subjectHexa-n-acetylchitohexaosees_ES
Keywordsdc.subjectTumor-ellses_ES
Keywordsdc.subjectCanceres_ES
Keywordsdc.subjectInflammasomees_ES
Keywordsdc.subjectMechanismses_ES
Keywordsdc.subjectExpressiones_ES
Keywordsdc.subjectEfficacyes_ES
Keywordsdc.subjectVaccinees_ES
Keywordsdc.subjectDeathes_ES
Títulodc.titleChitosan-based nanoparticles for Intracellular delivery of ISAV fusion protein cDNA into melanoma cells: a path to develop oncolytic anticancer therapieses_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorctces_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile