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Authordc.contributor.authorHurtado, Luz 
Authordc.contributor.authorMiranda, Claudio D. 
Authordc.contributor.authorRojas, Rodrigo 
Authordc.contributor.authorGodoy, Félix A. 
Authordc.contributor.authorAñazco, Mark A. 
Authordc.contributor.authorRomero Ormazábal, Jaime 
Admission datedc.date.accessioned2020-06-16T22:14:38Z
Available datedc.date.available2020-06-16T22:14:38Z
Publication datedc.date.issued2020
Cita de ítemdc.identifier.citationAnimals (2020); 10(3): 505es_ES
Identifierdc.identifier.other10.3390/ani10030505
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/175521
Abstractdc.description.abstractSimple Summary The culture of the marine fish red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibiotics, mainly florfenicol. In this study, the role of live prey (rotifers and the brine shrimp Artemia franciscana) used to feed fish larvae as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis was investigated. Samples of live feeds were collected during the larval growth period and their bacterial contents were determined. High levels of potentially opportunistic pathogens, such as Vibrio spp., as well as florfenicol-resistant bacteria, were detected. Sixty-five florfenicol-resistant isolates were recovered from these cultures and identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%), which exhibited a high incidence of co-resistance to the antibiotics streptomycin, oxytetracycline, co-trimoxazole, and kanamycin. The majority of them carried the florfenicol-resistance encoding genes floR and fexA. The high prevalence of antibiotic-resistant bacteria and the associated genetic elements in live feed administered to reared fish larvae requires the prompt implementation of efficient management strategies to prevent future therapy failures in fish larval cultures and the spread of antibiotic-resistant bacteria to associated aquatic environments. Abstract The culture of red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibacterials. The main aim of this study was to evaluate the role of live feed as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis. Samples of rotifer and Artemia cultures used as live feed were collected during the larval growth period and culturable bacterial counts were performed using a spread plate method. Rotifer and Artemia cultures exhibited high levels of resistant bacteria (8.03 x 10(4) to 1.79 x 10(7) CFU/g and 1.47 x 10(6) to 3.50 x 10(8) CFU/g, respectively). Sixty-five florfenicol-resistant isolates were identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%) using 16S rRNA gene sequence analysis. A high incidence of resistance to streptomycin (93.8%), oxytetracycline (89.2%), co-trimoxazole (84.6%), and kanamycin (73.8%) was exhibited by resistant isolates. A high proportion of isolates (76.9%) carried the florfenicol-resistance encoding genes floR and fexA, as well as plasmid DNA (75.0%). The high prevalence of multiresistant bacteria in live feed increases the incidence of the resistant microbiota in reared fish larvae, thus proper monitoring and management strategies for live feed cultures appear to be a priority for preventing future therapy failures in fish larval cultures.es_ES
Patrocinadordc.description.sponsorshipComisión Nacional de Investigación Cientifica y Tecnológica (CONICYT) 1171772 CORFO 15PTE-C47381 Comisión Nacional de Investigación Cientifica y Tecnológica (CONICYT) 21151482 Universidad de Los Lagoses_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherMDPIes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceAnimalses_ES
Keywordsdc.subjectResistant bacteriaes_ES
Keywordsdc.subjectRed cusk eeles_ES
Keywordsdc.subjectGenypterus chilensises_ES
Keywordsdc.subjectFlorfenicoles_ES
Keywordsdc.subjectVibrioses_ES
Keywordsdc.subjectLive feedes_ES
Keywordsdc.subjectRotiferes_ES
Keywordsdc.subjectArtemiaes_ES
Keywordsdc.subjectFloRes_ES
Keywordsdc.subjectFexAes_ES
Títulodc.titleLive Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)es_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorctces_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile