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Authordc.contributor.authorAraujo Garrido, Juan Luis 
Authordc.contributor.authorBaisón Olmo, Fernando 
Authordc.contributor.authorBernal Bayard, Joaquín 
Authordc.contributor.authorRomero, Francisco 
Authordc.contributor.authorRamos Morales, Francisco 
Admission datedc.date.accessioned2020-06-22T20:33:55Z
Available datedc.date.available2020-06-22T20:33:55Z
Publication datedc.date.issued2020
Cita de ítemdc.identifier.citationInt. J. Mol. Sci. 2020, 21, 3193es_ES
Identifierdc.identifier.other10.3390/ijms21093193
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/175627
Abstractdc.description.abstractSalmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase activity. We carried out a yeast two-hybrid screen and have identified tubulin-binding cofactor B (TBCB) as a new binding partner for this effector. SseK1 catalyzed the addition of N-acetylglucosamine to arginine on TBCB, and its expression promoted the stabilization of the microtubule cytoskeleton of HEK293T cells. The conserved Asp-x-Asp (DxD) motif that is essential for the activity of SseK1 was required for the binding and modification of TBCB and for the effect on the cytoskeleton. Our study has identified a novel target for SseK1 and suggests that this effector may have a role in the manipulation of the host cell microtubule network to provide a safe niche for this pathogen.es_ES
Patrocinadordc.description.sponsorshipSpanish Ministerio de Economia, Industria y Competitividad - Agencia Estatal de Investigacion European Union (EU) SAF2016-75365-R European Union (EU) 842629es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherMDPIes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceInternational Journal of Molecular Scienceses_ES
Keywordsdc.subjectTBCBes_ES
Keywordsdc.subjectSseK1es_ES
Keywordsdc.subjectTubulines_ES
Keywordsdc.subjectType III secretiones_ES
Keywordsdc.subjectSalmonellaes_ES
Keywordsdc.subjectArginine N-glycosyltransferasees_ES
Títulodc.titleTubulinfolding cofactor TBCB is a target of the salmonella effector protein SseK1es_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorapces_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile