Nanocoatings of Bovine Serum Albumin on Glass: Effects of pH and Temperature
Author
dc.contributor.author
Acuña Nelson, Sergio Miguel
Author
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Bastías Montes, José Miguel
Author
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Cerda Leal, Fabiola Rossana
Author
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Parra Flores, Julio Enrique
Author
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Aguirre García, Juan
Author
dc.contributor.author
Toledo, Pedro G.
Admission date
dc.date.accessioned
2020-10-21T19:26:25Z
Available date
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2020-10-21T19:26:25Z
Publication date
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2020
Cita de ítem
dc.identifier.citation
Journal of Nanomaterials Volume 2020, Article ID 8640818, 11 pages
es_ES
Identifier
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10.1155/2020/8640818
Identifier
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https://repositorio.uchile.cl/handle/2250/177276
Abstract
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Protein adsorption is influenced by many factors such as temperature, pH, protein size and structure, or surface energy and roughness, among others. Self-assembled monolayers (SAMs) and the Langmuir-Blodgett (LB) technique are two of the techniques more used to produces ultrathin films of proteins on surfaces. In this work, we established protocols for the preparation of nanocoatings of bovine serum albumin (BSA) protein on glass surface using SAMs and LB. Furthermore, we determined how small changes in temperature and pH can affect the covering when SAMs are used. Using a combination of different analyses, such as relative roughness, dynamic contact angles, and atomic force microscopy (AFM), it was possible to establish conditions to obtain a uniform nanocoating using SAMs. The results of the analysis of the nanocoating performed using the LB technique were very similar to those obtained using SAMs. The Derjaguin-Landau-Verwey-Overbeek (DLVO) theory in conjunction with the AFM images showed that electrostatic interactions are very important in the self-assembly process, but a process dominated solely by attraction is not sufficient to achieve a good SAM nanocoating, since it does not allow proper orientation and packaging of BSA molecules on the glass surface.
es_ES
Patrocinador
dc.description.sponsorship
Universidad del Bio-Bio
191520 4/R
GI 152322/EF
GI 195420 E/F
ANID/FONDAP/15130015