Synthesis of EPA- and DHA-enriched structured acylglycerols at the sn-2 position starting from commercial salmon oil by enzymatic lipase catalysis under supercritical conditions
Author
dc.contributor.author
Dovale Rosabal, Gretel
Author
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Rodríguez Melis, Alicia
Author
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Espinosa Escalona, Berta Alejandra
Author
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Barriga Pinto, Gonzalo Andrés
Author
dc.contributor.author
Aubourg Martín, Santiago Pedro
Admission date
dc.date.accessioned
2021-10-25T21:00:30Z
Available date
dc.date.available
2021-10-25T21:00:30Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
Molecules 2021, 26, 3094
es_ES
Identifier
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10.3390/molecules26113094
Identifier
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https://repositorio.uchile.cl/handle/2250/182390
Abstract
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There is consistent evidence that long-chain polyunsaturated fatty acids (LCPUFA) belonging to the n-3 series, i.e., eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) acids, decrease the risk of heart, circulatory and inflammatory diseases. Furthermore, the bioavailability of such fatty acids has been shown to depend on their location in triacylglycerol (TG) molecules at the sn-2 position. Consequently, great attention has been accorded to the synthesis of structured acylglycerols (sAG), which include EPA or DHA at the sn-2 position. The aim of this work was to synthesize sAG starting from deodorized refined commercial salmon oil. For this, immobilized lipase B from Candida antarctica (nonspecific) was used as a catalyst for the intra-interesterification process under CO2 supercritical conditions (CO2SC). According to the CO2SC reaction time, three different fractions including sAG compounds were obtained. The location of EPA and DHA at the sn-2 position in the resulting glycerol backbone was identified by mass spectrometry (MALDI-TOF) analysis. In all fractions obtained, a marked decrease in the starting TG content was observed, while an increase in the DHA content at the sn-2 position was detected. The fraction obtained after the longest reaction time period (2 h) led to the highest yield of sn-2 position DHA in the resulting sAG molecule.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1181774
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
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MDPI
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States
Synthesis of EPA- and DHA-enriched structured acylglycerols at the sn-2 position starting from commercial salmon oil by enzymatic lipase catalysis under supercritical conditions