The multi metal-resistant bacterium Cupriavidus metallidurans CH34 affects growth and metal mobilization in Arabidopsis thaliana plants exposed to copper
Author
dc.contributor.author
Clavero León, Claudia Fernanda
Author
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Ruiz, Daniela
Author
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Cillero, Javier
Author
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Orlando, Julieta Laura
Author
dc.contributor.author
González, Bernardo
Admission date
dc.date.accessioned
2021-11-29T21:49:23Z
Available date
dc.date.available
2021-11-29T21:49:23Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
PEERJ (2021) Volumen 9, Artículo 11373
es_ES
Identifier
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10.7717/peerj.11373
Identifier
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https://repositorio.uchile.cl/handle/2250/182934
Abstract
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Copper (Cu) is important for plant growth, but high concentrations can lead to
detrimental effects such as primary root length inhibition, vegetative tissue chlorosis,
and even plant death. The interaction between plant-soil microbiota and roots can
potentially affect metal mobility and availability, and, therefore, overall plant metal
concentration. Cupriavidus metallidurans CH34 is a multi metal-resistant bacterial
model that alters metal mobility and bioavailability through ion pumping, metal
complexation, and reduction processes. The interactions between strain CH34 and
plants may affect the growth, metal uptake, and translocation of Arabidopsis thaliana
plants that are exposed to or not exposed to Cu. In this study, we looked also at the
specific gene expression changes in C. metallidurans when co-cultured with Cu-exposed
A. thaliana. We found that A. thaliana's rosette area, primary and secondary root
growth, and dry weight were affected by strain CH34, and that beneficial or detrimental
effects depended on Cu concentration. An increase in some plant growth parameters
was observed at copper concentrations lower than 50 mM and significant detrimental
effects were found at concentrations higher than 50 mM Cu. We also observed up to a
90% increase and 60% decrease in metal accumulation and mobilization in inoculated
A. thaliana. In turn, copper-stressed A. thaliana altered C. metallidurans colonization,
and cop genes that encoded copper resistance in strain CH34 were induced by the
combination of A. thaliana and Cu. These results reveal the complexity of the plantbacteria-
metal triad and will contribute to our understanding of their applications in
plant growth promotion, protection, and phytoremediation strategies.
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Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1151130
1190634
ANID PIA/BASAL FB0002
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Lenguage
dc.language.iso
en
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Publisher
dc.publisher
Peerj
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States
The multi metal-resistant bacterium Cupriavidus metallidurans CH34 affects growth and metal mobilization in Arabidopsis thaliana plants exposed to copper