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La pesca artesanal y la acuicultura
(Universidad de Chile, 2000)
establecido en nuestra Constitución y leyes correspondientes, su regulación en el derecho comparado, y, principalmente, un análisis a la actual Ley de Pesca y Acuicultura, sobretodo, en cuanto a las normas que afectan directa o indirectamente a este sector...
Máquina trasladable para el proceso de graduación in-situ de turbot cultivado en estanque.
(Universidad de Chile, 2005)
Sistema de Control de Inventario de Peces Vivos para la Industria almonicultora
(Universidad de ChilePrograma Cybertesis, 2009)
CPAA Centro de producción acuícola artesanal Caleta San Marcos Iquique
(Universidad de Chile, 2010)
podemos dividir
la producción pesquera en tres áreas, la industrial, la artesanal y la acuicultura. según
los dartos de sernapesca correspondientes al periódo de xxx a xxx podemos ver que en
el contexto chileno la pesca industrial representa el 43...
Oportunidades en Latinoamérica Relacionadas con el Mercado de la Acuicultura
(Universidad de Chile, 2009)
La industria productora de tilapia Costarricense y Hondureña fue estudiada para determinar
su potencial productivo y atractivo de mercado para PHARMAQ AS, empresa farmacéutica
veterinaria Noruega con foco en acuicultura que se caracteriza por la...
Encapsulación de fagos en perlas de alginato para el delivery de fagoterapia en acuicultura contra vibrios
(Universidad de Chile, 2023)
La acuicultura en Chile es una industria importante y en constante crecimiento, contando con múltiples especies acuáticas como productos. Sin embargo, uno de los desafíos que enfrenta la acuicultura es el control de enfermedades que afectan a los...
Aquaculture in Chile is an important and constantly growing industry, with multiple aquatic species as a product. However, one of the challenges facing aquaculture is the control of diseases affecting cultured organisms, where vibriosis is one of the most recurrent bacterial diseases, especially in larval stages. Vibrio alginolyticus is a Vibrio that affects a wide range of hosts in aquaculture in Chile, especially in conditions of high culture densities and contamination by organic waste. Currently, the management of vibriosis and other bacterial infections is based on environmental control and frequent use of antibiotics. However, the excessive use of antibiotics has led to an increase in the frequency of selection of resistant bacteria, encouraging the generation of alternative strategies. Phage therapy has gained renewed interest, with the use of bacteriophages as agents for the control of pathogenic bacteria, due to their ability to infect and lyse specific host bacteria. However, the susceptibility of phages to external agents hinders the development of this technique as an antibacterial therapy by presenting disadvantages when administering and storing bacteriophages. The objective of the present study was to design alginate beads for the delivery of phages against vibrios in aquaculture. In order to complete this goal, we started with the characterization of the phages previously isolated in the Biotechnology laboratory of INTA. There were 8 phages with the ability to lyse the pathogenic strain GV09 (V. alginolyticus), where only 2 of the 8 phages were found to be highly virulent due to their EOP. These were the phages Ch20 and Ca8 that presented very similar characterization, transparent lysis, double-stranded DNA, as well as similar adsorption and latency times. However, a DGREA analysis revealed that the phages are genetically different. Then we proceeded with the analysis of infection kinetics of the phage with a higher Burst Size, defined as the number of virions released per infected bacterium, corresponding to phage Ch20. A reduction of bacterial growth was observed by absorbance and CFU/ml count. Finally, the phage was encapsulated under 3 conditions of final % of alginate (% v/v) in solution to determine the % that allowed the highest rate of phage diffusion to the medium. As a result, the beads at 2% final alginate (% v/v) in solution presented a higher rate of diffusion after 5 hours of testing with respect to the beads at 3%. Therefore, the 2% beads were selected to determine the viability of the phage under different storage conditions, with respect to the temperature used. A better stability of the encapsulated phage was obtained within the first 15 days at 4 °C, and in the case of the non-encapsulated phages, after 30 days of storage, it presented a better stability of its titer at -80 °C. Therefore, the results show the potential of phage encapsulation in alginate beads for phage delivery and storage in aquaculture....
Aquaculture in Chile is an important and constantly growing industry, with multiple aquatic species as a product. However, one of the challenges facing aquaculture is the control of diseases affecting cultured organisms, where vibriosis is one of the most recurrent bacterial diseases, especially in larval stages. Vibrio alginolyticus is a Vibrio that affects a wide range of hosts in aquaculture in Chile, especially in conditions of high culture densities and contamination by organic waste. Currently, the management of vibriosis and other bacterial infections is based on environmental control and frequent use of antibiotics. However, the excessive use of antibiotics has led to an increase in the frequency of selection of resistant bacteria, encouraging the generation of alternative strategies. Phage therapy has gained renewed interest, with the use of bacteriophages as agents for the control of pathogenic bacteria, due to their ability to infect and lyse specific host bacteria. However, the susceptibility of phages to external agents hinders the development of this technique as an antibacterial therapy by presenting disadvantages when administering and storing bacteriophages. The objective of the present study was to design alginate beads for the delivery of phages against vibrios in aquaculture. In order to complete this goal, we started with the characterization of the phages previously isolated in the Biotechnology laboratory of INTA. There were 8 phages with the ability to lyse the pathogenic strain GV09 (V. alginolyticus), where only 2 of the 8 phages were found to be highly virulent due to their EOP. These were the phages Ch20 and Ca8 that presented very similar characterization, transparent lysis, double-stranded DNA, as well as similar adsorption and latency times. However, a DGREA analysis revealed that the phages are genetically different. Then we proceeded with the analysis of infection kinetics of the phage with a higher Burst Size, defined as the number of virions released per infected bacterium, corresponding to phage Ch20. A reduction of bacterial growth was observed by absorbance and CFU/ml count. Finally, the phage was encapsulated under 3 conditions of final % of alginate (% v/v) in solution to determine the % that allowed the highest rate of phage diffusion to the medium. As a result, the beads at 2% final alginate (% v/v) in solution presented a higher rate of diffusion after 5 hours of testing with respect to the beads at 3%. Therefore, the 2% beads were selected to determine the viability of the phage under different storage conditions, with respect to the temperature used. A better stability of the encapsulated phage was obtained within the first 15 days at 4 °C, and in the case of the non-encapsulated phages, after 30 days of storage, it presented a better stability of its titer at -80 °C. Therefore, the results show the potential of phage encapsulation in alginate beads for phage delivery and storage in aquaculture....
Evaluación de la eficacia de productos comerciales no tradicionales (Supratect ™ y Cleanmix) como tratamiento en ovas de S. salar desafiadas con Saprolegnia spp.
(Universidad de Chile, 2022)
La saprolegniasis es una patología producida por Saprolegnia spp, género Oomycetes, de gran importancia a nivel nacional y mundial debido a las pérdidas que genera en los centros de acuicultura, afectando desde ovas a peces adultos.
Actualmente, lo...
Saprolegniasis is a pathology caused by Saprolegnia spp., genus Oomycetes, of great importance at national and global level due to the losses it generates in aquaculture centers, affecting from eggs to adult fish. Currently, the most used products for its control are Formalin and Hydrogen Peroxide, showing good results. However, their adverse effects complicate their use in certain stages of the development of Salmo salar and Formalin is considered as a product not suitable for use in aquaculture. Two products that do not have toxic components, with natural ingredients, and are not harmful to people, fish, or the environment, Supratect ™ and Cleanmix, were tested for their effectiveness to control de agent. Two hundred and four eggs of S. salar were used, arranged in 6 groups (Negative Control, Positive Control, Formalin, Hydrogen Peroxide, Supratect ™ and Cleanmix), of 34 individuals each, which were subjected to Saprolegnia infestation by inoculum (except negative control) and subsequently treated with traditional and non-traditional compounds (except negative and positive control). The results showed an equivalence between traditional treatments (Formalin and Hydrogen Peroxide; 59% and 62% survival) and non-traditional treatments (Supratect ™ and Cleanmix; 62% and 56% survival). Larger-scale trials are suggested to evaluate their use in conjunction with traditional products to reduce their use, or even promote synergy between the two...
Saprolegniasis is a pathology caused by Saprolegnia spp., genus Oomycetes, of great importance at national and global level due to the losses it generates in aquaculture centers, affecting from eggs to adult fish. Currently, the most used products for its control are Formalin and Hydrogen Peroxide, showing good results. However, their adverse effects complicate their use in certain stages of the development of Salmo salar and Formalin is considered as a product not suitable for use in aquaculture. Two products that do not have toxic components, with natural ingredients, and are not harmful to people, fish, or the environment, Supratect ™ and Cleanmix, were tested for their effectiveness to control de agent. Two hundred and four eggs of S. salar were used, arranged in 6 groups (Negative Control, Positive Control, Formalin, Hydrogen Peroxide, Supratect ™ and Cleanmix), of 34 individuals each, which were subjected to Saprolegnia infestation by inoculum (except negative control) and subsequently treated with traditional and non-traditional compounds (except negative and positive control). The results showed an equivalence between traditional treatments (Formalin and Hydrogen Peroxide; 59% and 62% survival) and non-traditional treatments (Supratect ™ and Cleanmix; 62% and 56% survival). Larger-scale trials are suggested to evaluate their use in conjunction with traditional products to reduce their use, or even promote synergy between the two...
Complejo acuícola Arenas Blancas. Arquitectura, sustentabilidad, patrimonio
(Universidad de Chile, 2013)