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Authordc.contributor.authorArmas, Pablo 
Authordc.contributor.authorCachero, Sebastián es_CL
Authordc.contributor.authorLombardo, Verónica A. es_CL
Authordc.contributor.authorWeiner, Andrea es_CL
Authordc.contributor.authorAllende Connelly, Miguel es_CL
Authordc.contributor.authorCalcaterra, Nora B. es_CL
Admission datedc.date.accessioned2007-04-27T15:42:38Z
Available datedc.date.available2007-04-27T15:42:38Z
Publication datedc.date.issued2004-08-04
Cita de ítemdc.identifier.citationGENE 337: 151-161 AUG 4 2004en
Identifierdc.identifier.issn0378-1119
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/118589
Abstractdc.description.abstractHere we analyse the structural organisation and expression of the zebrafish cellular nucleic acid-binding protein (zCNBP) gene and protein. The gene is organised in five exons and four introns. A noteworthy feature of the gene is the absence of a predicted promoter region. The coding region encodes a 163-amino acid polypeptide with the highly conserved general structural organisation of seven CCHC Zn knuckle domains and an RGG box between the first and the second Zn knuckles. Although theoretical alternative splicing is possible, only one form of zCNBP is actually detected. This form is able to bind to single-stranded DNA and RNA probes in vitro. The analysis of zCNBP developmental expression shows a high amount of CNBP-mRNA in ovary and during the first developmental stages. CNBP-mRNA levels decrease while early development progresses until the midblastula transition (MBT) stage and increases again thereafter. The protein is localised in the cytoplasm of blastomeres whereas it is mainly nuclear in developmental stages after the MBT. These findings suggest that CNBP is a strikingly conserved single-stranded nucleic acid-binding protein which might interact with maternal mRNA during its storage in the embryo cell cytoplasm. It becomes nuclear once MBT takes place possibly in order to modulate zygotic transcription and/or to associate with newly synthesised transcripts.en
Lenguagedc.language.isoenen
Publisherdc.publisherELSEVIERen
Keywordsdc.subjectZINC-FINGER PROTEINen
Títulodc.titleZebrafish cellular nucleic acid-binding protein: gene structure and developmental behaviouren
Document typedc.typeArtículo de revista


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