Author | dc.contributor.author | Ugarte, G. | |
Author | dc.contributor.author | Delgado Arriagada, Ricardo | es_CL |
Author | dc.contributor.author | O'Day, P.M. | es_CL |
Author | dc.contributor.author | Farjah, F. | es_CL |
Author | dc.contributor.author | Cid, L. P. | es_CL |
Author | dc.contributor.author | Vergara Montecinos, Cecilia | es_CL |
Author | dc.contributor.author | Bacigalupo Vicuña, Juan | es_CL |
Admission date | dc.date.accessioned | 2007-05-17T20:00:12Z | |
Available date | dc.date.available | 2007-05-17T20:00:12Z | |
Publication date | dc.date.issued | 2005-10 | |
Cita de ítem | dc.identifier.citation | OURNAL OF MEMBRANE BIOLOGY | en |
Identifier | dc.identifier.issn | 0022-2631 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/118619 | |
Abstract | dc.description.abstract | We report that Drosohhila retinal photoreceptors express inwardly rectifying chloride channels that seem to be orthologous to mammalian CIC-2 inward rectifier channels. We measured inwardly rectifying Cl- currents in photoreceptor plasma membranes: Hyperpolarization under whole-cell tight-seal voltage clamp induced inward Cl- currents; and hyperpolarization of voltage-clamped inside-out patches excised from plasma membrane induced CI- currents that have a unitary channel conductance of similar to 3.7 pS. The channel was inhibited by 1 mM Zn2+ and by 1 mM 9-anthracene, but was insensitive to DIDS. Its anion permeability sequence is Cl- = SCN- > Br- > > I-, characteristic of CIC-2 channels. Exogenous polyunsaturated fatty acid, linolenic acid, enhanced or activated the inward rectifier Cl- currents in both whole-cell and excised patch-clamp recordings. Using RT-PCR, we found expression in Drosophila retina of a CIC-2 gene orthologous to mammalian CIC-2 channels. Antibodies to rat CIC-2 channels labeled Drosophila photoreceptor plasma membranes and synaptic regions. Our results provide evidence that the inward rectification in Drosophila retinal photoreceptors is mediated by CIC-2-like channels in the non-transducing (extra-rhabdomeral) plasma membrane, and that this inward rectification can be modulated by polyunsatturated fatty acid. | en |
Lenguage | dc.language.iso | en | en |
Publisher | dc.publisher | SPRINGER | en |
Keywords | dc.subject | LIGHT-SENSITIVE CHANNELS | en |
Título | dc.title | Putative ClC-2 chloride channel mediates inward rectification in Drosophila retinal photoreceptors | en |
Document type | dc.type | Artículo de revista | |