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Authordc.contributor.authorAlcaíno Gorman, Jennifer 
Authordc.contributor.authorBarahona‑Crisóstomo, Salvador es_CL
Authordc.contributor.authorCarmona, Marisela es_CL
Authordc.contributor.authorLozano, Carla es_CL
Authordc.contributor.authorMarcoleta, Andrés es_CL
Authordc.contributor.authorNiklitschek, Mauricio es_CL
Authordc.contributor.authorSepúlveda, Dionisia es_CL
Authordc.contributor.authorBaeza Cancino, Marcelo es_CL
Authordc.contributor.authorCifuentes Guzmán, Víctor es_CL
Admission datedc.date.accessioned2010-01-06T14:07:28Z
Available datedc.date.available2010-01-06T14:07:28Z
Publication datedc.date.issued2008-10-06
Cita de ítemdc.identifier.citationBMC MICROBIOLOGY Volume: 8 Article Number: 169 Published: OCT 6 2008en_US
Identifierdc.identifier.issn1471-2180
Identifierdc.identifier.other10.1186/1471-2180-8-169
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/118897
Abstractdc.description.abstractBackground: The yeast Xanthophyllomyces dendrorhous synthesizes astaxanthin, a carotenoid with high commercial interest. The proposed biosynthetic route in this organism is isopentenylpyrophosphate (IPP) -> geranyleranyl pyrophosphate (GGPP) -> phytoene -> lycopene -> beta-carotene -> astaxanthin. Recently, it has been published that the conversion of beta-carotene into astaxanthin requires only one enzyme, astaxanthin synthase or CrtS, encoded by crtS gene. This enzyme belongs to the cytochrome P450 protein family. Results: In this work, a crtR gene was isolated from X. dendrorhous yeast, which encodes a cytochrome P450 reductase (CPR) that provides CrtS with the necessary electrons for substrate oxygenation. We determined the structural organization of the crtR gene and its location in the yeast electrophoretic karyotype. Two transformants, CBSTr and T13, were obtained by deleting the crtR gene and inserting a hygromycin B resistance cassette. The carotenoid composition of the transformants was altered in relation to the wild type strain. CBSTr forms yellow colonies because it is unable to produce astaxanthin, hence accumulating beta-carotene. T13 forms pale colonies because its astaxanthin content is reduced and its beta-carotene content is increased. Conclusion: In addition to the crtS gene, X. dendrorhous requires a novel gene, crtR, for the conversion of beta-carotene to astaxanthin.en_US
Patrocinadordc.description.sponsorshipThis work was supported by U. de Chile ENL 08/06 and: MECESUP UCH 0106 by graduate scholarships to JA and MN, Fundación María Ghilardi by a graduate scholarship to CL, CONICYT by a graduate scholarship to AM.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherBIOMED CENTRAL LTDen_US
Keywordsdc.subjectYEAST PHAFFIA-RHODOZYMAen_US
Títulodc.titleCloning of the cytochrome p450 reductase (crtR) gene and its involvement in the astaxanthin biosynthesis of Xanthophyllomyces dendrorhousen_US
Document typedc.typeArtículo de revista


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