Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco, a high altitude saline wetland in northern Chile
Author | dc.contributor.author | Dorador, Cristina | |
Author | dc.contributor.author | Busekow, Annika | es_CL |
Author | dc.contributor.author | Vila Pinto, Irma | es_CL |
Author | dc.contributor.author | Imhoff, Johannes F. | es_CL |
Author | dc.contributor.author | Witzel, Karl-Paul | es_CL |
Admission date | dc.date.accessioned | 2010-01-15T18:36:27Z | |
Available date | dc.date.available | 2010-01-15T18:36:27Z | |
Publication date | dc.date.issued | 2008-05 | |
Cita de ítem | dc.identifier.citation | EXTREMOPHILES Volume: 12 Issue: 3 Pages: 405-414 Published: MAY 2008 | en_US |
Identifier | dc.identifier.issn | 1431-0651 | |
Identifier | dc.identifier.other | 10.1007/s00792-008-0146-x | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/118936 | |
Abstract | dc.description.abstract | We analyzed enrichment cultures of ammonia-oxidizing bacteria (AOB) collected from different areas of Salar de Huasco, a high altitude, saline, pH-neutral water body in the Chilean Altiplano. Samples were inoculated into mineral media with 10 mM NH4+ at five different salt concentrations (10, 200, 400, 800 and 1,400 mM NaCl). Low diversity (up to three phylotypes per enrichment) of beta-AOB was detected using 16S rDNA and amoA clone libraries. Growth of beta-AOB was only recorded in a few enrichment cultures and varied according to site or media salinity. In total, five 16S rDNA and amoA phylotypes were found which were related to Nitrosomonas europaea/Nitrosococcus mobilis, N. marina and N. communis clusters. Phylotype 1-16S was 97% similar with N. halophila, previously isolated from Mongolian soda lakes, and phylotypes from amoA sequences were similar with yet uncultured beta-AOB from different biofilms. Sequences related to N. halophila were frequently found at all salinities. Neither gamma-AOB nor ammonia-oxidizing Archaea were recorded in these enrichment cultures. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | SPRINGER TOKYO | en_US |
Keywords | dc.subject | 16S RIBOSOMAL-RNA | en_US |
Título | dc.title | Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco, a high altitude saline wetland in northern Chile | en_US |
Document type | dc.type | Artículo de revista |
Files in this item
This item appears in the following Collection(s)
-
Artículos de revistas
Artículos de revistas