| Author | dc.contributor.author | Morales, Rodrigo | |
| Author | dc.contributor.author | Buytaert-Hoefen, Kimberley A. | es_CL |
| Author | dc.contributor.author | González Romero, Dennisse | es_CL |
| Author | dc.contributor.author | Castilla, Joaquín | es_CL |
| Author | dc.contributor.author | Hansen, Eric T. | es_CL |
| Author | dc.contributor.author | Hlavinka, Dennis | es_CL |
| Author | dc.contributor.author | Goodrich, Raymond P. | es_CL |
| Author | dc.contributor.author | Soto, Claudio | es_CL |
| Admission date | dc.date.accessioned | 2010-01-18T19:54:28Z | |
| Available date | dc.date.available | 2010-01-18T19:54:28Z | |
| Publication date | dc.date.issued | 2008-12-12 | |
| Cita de ítem | dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 377 Issue: 2 Pages: 373-378 Published: DEC 12 2008 | en_US |
| Identifier | dc.identifier.issn | 0006-291X | |
| Identifier | dc.identifier.other | 10.1016/j.bbrc.2008.09.141 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/118950 | |
| Abstract | dc.description.abstract | The link between a new variant form of Creutzfeldt-Jakob disease (vCJD) and the consumption of prion contaminated cattle meat as well as recent findings showing that vCJD can be transmitted by blood transfusion have raised public health concerns. Currently, a reliable test to identify prions in blood samples is not available. The purpose of this study was to evaluate the possibility to remove scrapie prion protein (PrPSc) and infectivity from red blood cell (RBC) suspensions by a simple washing procedure using a cell separation and washing device. The extent of prion removal was assessed by Western blot, PMCA and infectivity bioassays. Our results revealed a substantial removal of infectious prions (>= 3 logs of infectivity) by all techniques used. These data suggest that a significant amount of infectivity present in RBC preparations can be removed by a simple washing procedure. This technology may lead to increased safety of blood products and reduce the risk of further propagation of prion diseases. | en_US |
| Patrocinador | dc.description.sponsorship | This work was supported
by NIH Grant NS049173 and a grant
from CaridianBCT Biotechnologies LLC to C.S. and by a project
from
the Department
of Defense to CaridianBCT LLC. | en_US |
| Lenguage | dc.language.iso | en | en_US |
| Publisher | dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
| Keywords | dc.subject | TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHY | en_US |
| Título | dc.title | Reduction of prion infectivity in packed red blood cells | en_US |
| Document type | dc.type | Artículo de revista | |
