Author | dc.contributor.author | Castillo, Karen | |
Author | dc.contributor.author | Restrepo, Diego | es_CL |
Author | dc.contributor.author | Bacigalupo Vicuña, Juan | es_CL |
Admission date | dc.date.accessioned | 2010-11-17T19:08:24Z | |
Available date | dc.date.available | 2010-11-17T19:08:24Z | |
Publication date | dc.date.issued | 2010 | |
Cita de ítem | dc.identifier.citation | European Journal of Neuroscience, Vol. 32, pp. 932–938, 2010 | en_US |
Identifier | dc.identifier.other | doi:10.1111/j.1460-9568.2010.07393.x | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119093 | |
Abstract | dc.description.abstract | Signal transduction depends critically on the spatial localization of protein constituents. A key question in odor transduction is whether
chemotransduction proteins organize into discrete molecular complexes throughout olfactory cilia or distribute homogeneously along
the ciliary membrane. Our recordings of Ca2+ changes in individual cilia with unprecedented spatial and temporal resolution, by the
use of two-photon microscopy, provide solid evidence for Ca2+ microdomains (transducisomes). Dissociated frog olfactory neurons
were preloaded with caged-cAMP and fluo-4 acetoxymethyl ester probe Ca2+ indicator. Ca2+ influx through cyclic nucleotide-gated
(CNG) channels was evoked by uniformly photoreleasing cAMP, while ciliary Ca2+ was measured. Discrete fluorescence events were
clearly resolved. Events were missing in the absence of external Ca2+, consistent with the absence of internal Ca2+ sources.
Fluorescence events at individual microdomains resembled single-CNG channel fluctuations in shape, mean duration and kinetics,
indicating that transducisomes typically contain one to three CNG channels. Inhibiting the Na+ ⁄ Ca2+ exchanger or the Ca2+-ATPase
prolonged the decay of evoked intraciliary Ca2+ transients, supporting the participation of both transporters in ciliary Ca2+ clearance,
and suggesting that both molecules localize close to the CNG channel. Chemosensory transducisomes provide a physical basis for
the low amplification and for the linearity of odor responses at low odor concentrations. | en_US |
Patrocinador | dc.description.sponsorship | This work was supported by grant TW007920 from the
Fogarty International Center of the NIH (J. Bacigalupo and D. Restrepo),
NIDCD grants DC006070 and DC04657 (D. Restrepo), MIDEPLAN ICMP05-
001-F (J. Bacigalupo), FONDECYT 1080653 (J. Bacigalupo), a MECESUP
UCH0409 training research fellowship (K. Castillo), the Graduate
Department and Academic Affairs, University of Chile (K, Castillo) and a
CONICYT doctoral fellowship (K. Castillo). | en_US |
Lenguage | dc.language.iso | en | en_US |
Keywords | dc.subject | Ca2+ fluorescence | en_US |
Título | dc.title | Cellular & molecular Ca2+ microdomains in olfactory cilia support low signaling amplification of odor transduction | en_US |
Document type | dc.type | Artículo de revista | |