Show simple item record

Authordc.contributor.authorFeijóo, Carmen Gloria 
Authordc.contributor.authorManzanares, Miguel es_CL
Authordc.contributor.authorCalle Mustienes, Elisa de la es_CL
Authordc.contributor.authorGómez Skarmeta, José Luis es_CL
Authordc.contributor.authorAllende Connelly, Miguel es_CL
Admission datedc.date.accessioned2011-03-29T17:25:02Z
Available datedc.date.available2011-03-29T17:25:02Z
Publication datedc.date.issued2004-04-03
Cita de ítemdc.identifier.citationDEVELOPMENT GENES AND EVOLUTION, Volume: 214, Issue: 6, Pages: 277-284, 2004en_US
Identifierdc.identifier.issn0949-944X
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/119117
Abstractdc.description.abstractGenes of the iroquois (Iro/Irx) family are highly conserved from Drosophila to mammals and they have been implicated in a number of developmental processes. In flies, the Iro genes participate in patterning events in the early larva and in imaginal disk specification. In vertebrates, the Irx genes regulate developmental events during gastrulation, nervous system regionalization, activation of proneural genes and organ patterning. The Iro genes in Drosophila and the Irx genes of mammals show a clustered organization in the genome. Flies have a single cluster comprising three genes while mammals have two clusters also having three genes each. Moreover, experimental evidence in flies shows that transcriptional regulatory elements are shared among genes within the Iro cluster, suggesting that the same may be true in vertebrates. To date, the genomic organization of the Irx genes in non-mammalian species has not been studied. In this work, we have isolated the irx5b gene from zebrafish, Danio rerio, and have characterized its expression pattern. Furthermore, we have identified the complete set of Irx genes in two fish species, the zebrafish and pufferfish, Takifugu rubripes, and have determined the genomic organization of these genes. Our analysis indicates that early in fish evolutionary history, the Irx gene clusters have been duplicated and that subsequent events have maintained the clustered organization for some of the genes, while others have been lost. In total there are 11 existing Irx genes in zebrafish and 10 in pufferfish. We propose a new nomenclature for the zebrafish Irx genes based on the analysis of their sequences and their genomic relationships.en_US
Patrocinadordc.description.sponsorshipWe wish to thank Gabino S nchez-P rez for help with phylogenetic analysis. Carolina Achondo and Florencio Espinoza provided technical help. C.G.F. and M.A. were supported by grants from Fondecyt (1031003), the ICM (P02-050) and by a grant from the UNAB (DI 10-03). M.M. and J.L.G.-S. were supported by grants from the Spanish Ministry of Science and Technology (BMC2002-03558 and BMC2001-2122, respectively). In addition, M.A. and J.L.G.-S. were supported by a CONICYT-CSIC collaboration grant (2003CL00021).en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherSPRINGERen_US
Keywordsdc.subjectIroquoisen_US
Títulodc.titleThe Irx gene family in zebrafish: genomic structure, evolution and initial characterization of irx5ben_US
Document typedc.typeArtículo de revistaen_US


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record