Author | dc.contributor.author | Mackenzie, Natalia | |
Author | dc.contributor.author | Brito, Mónica | es_CL |
Author | dc.contributor.author | Reyes, Ariel E. | es_CL |
Author | dc.contributor.author | Allende Connelly, Miguel | es_CL |
Admission date | dc.date.accessioned | 2011-04-04T12:26:52Z | |
Available date | dc.date.available | 2011-04-04T12:26:52Z | |
Publication date | dc.date.issued | 2003-11-24 | |
Cita de ítem | dc.identifier.citation | GENE, Volume: 328, Pages: 113-120, 2004 | en_US |
Identifier | dc.identifier.issn | 0378-1119 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119131 | |
Abstract | dc.description.abstract | The high-affinity copper transporter 1 (Ctr1) is a highly conserved transmembrane protein that mediates the internalization of copper
ions from the extracellular medium. In this study, we have isolated the zebrafish ctr1 gene. The zebrafish ctr1 cDNA encodes a protein
with 69% identity to the human orthologue and shows conservation of specific amino acid residues involved in copper transport. We find
only a single ctr1 gene in the zebrafish genome which maps to linkage group 5. The genomic structure of the zebrafish gene shows that it
consists of five exons and that exon–intron boundaries are absolutely conserved with the mammalian ctr1 genes. Expression in embryos
was analyzed by reverse transcription–polymerase chain reaction (RT–PCR) and by in situ hybridization. Zebrafish ctr1 is maternally
loaded, and transcripts can be detected throughout development and in adult fish. Distribution of ctr1 message appears ubiquitous during
early stages becoming restricted to the brain and ventral tissues by 24 h post fertilization (hpf). Beginning at 3 days post fertilization (dpf),
expression is found mainly in the developing intestine. Specific knockdown of ctr1 by antisense morpholino oligonucleotides (MOs)
causes early larval lethality. Defects include cell death in tissues where ctr1 is most heavily expressed, a finding similar to that described
for a mouse knockout of mCtr1. Despite the existence of at least one other copper transport mechanism in the fish, our studies show that
zebrafish ctr1 is an essential gene for development. | en_US |
Patrocinador | dc.description.sponsorship | MA was supported
by a grant from the International Copper Association and
by the Millennium Scientific Initiative (ICM P99-137-F).
AR was supported by a postdoctoral fellowship from
Conicyt (3010065). | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | ELSEVIER SCIENCE BV | en_US |
Keywords | dc.subject | Copper homeostasis | en_US |
Título | dc.title | Cloning, expression pattern and essentiality of the high-affinity copper transporter 1 (ctr1) gene in zebrafish | en_US |
Document type | dc.type | Artículo de revista | |