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Authordc.contributor.authorMackenzie, Natalia 
Authordc.contributor.authorBrito, Mónica es_CL
Authordc.contributor.authorReyes, Ariel E. es_CL
Authordc.contributor.authorAllende Connelly, Miguel es_CL
Admission datedc.date.accessioned2011-04-04T12:26:52Z
Available datedc.date.available2011-04-04T12:26:52Z
Publication datedc.date.issued2003-11-24
Cita de ítemdc.identifier.citationGENE, Volume: 328, Pages: 113-120, 2004en_US
Identifierdc.identifier.issn0378-1119
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/119131
Abstractdc.description.abstractThe high-affinity copper transporter 1 (Ctr1) is a highly conserved transmembrane protein that mediates the internalization of copper ions from the extracellular medium. In this study, we have isolated the zebrafish ctr1 gene. The zebrafish ctr1 cDNA encodes a protein with 69% identity to the human orthologue and shows conservation of specific amino acid residues involved in copper transport. We find only a single ctr1 gene in the zebrafish genome which maps to linkage group 5. The genomic structure of the zebrafish gene shows that it consists of five exons and that exon–intron boundaries are absolutely conserved with the mammalian ctr1 genes. Expression in embryos was analyzed by reverse transcription–polymerase chain reaction (RT–PCR) and by in situ hybridization. Zebrafish ctr1 is maternally loaded, and transcripts can be detected throughout development and in adult fish. Distribution of ctr1 message appears ubiquitous during early stages becoming restricted to the brain and ventral tissues by 24 h post fertilization (hpf). Beginning at 3 days post fertilization (dpf), expression is found mainly in the developing intestine. Specific knockdown of ctr1 by antisense morpholino oligonucleotides (MOs) causes early larval lethality. Defects include cell death in tissues where ctr1 is most heavily expressed, a finding similar to that described for a mouse knockout of mCtr1. Despite the existence of at least one other copper transport mechanism in the fish, our studies show that zebrafish ctr1 is an essential gene for development.en_US
Patrocinadordc.description.sponsorshipMA was supported by a grant from the International Copper Association and by the Millennium Scientific Initiative (ICM P99-137-F). AR was supported by a postdoctoral fellowship from Conicyt (3010065).en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherELSEVIER SCIENCE BVen_US
Keywordsdc.subjectCopper homeostasisen_US
Títulodc.titleCloning, expression pattern and essentiality of the high-affinity copper transporter 1 (ctr1) gene in zebrafishen_US
Document typedc.typeArtículo de revista


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