Mostrar el registro sencillo del ítem
Isolation, characterization and long term preservation of mutant strains of Xanthophyllomyces dendrorhous
Autor | dc.contributor.author | Baeza Cancino, Marcelo | |
Autor | dc.contributor.author | Retamales Molina, Patricio | es_CL |
Autor | dc.contributor.author | Sepúlveda, Dionisia | es_CL |
Autor | dc.contributor.author | Lodato, Patricia | es_CL |
Autor | dc.contributor.author | Jiménez, Antonio | es_CL |
Autor | dc.contributor.author | Cifuentes Guzmán, Víctor | es_CL |
Fecha ingreso | dc.date.accessioned | 2011-04-04T11:47:43Z | |
Fecha disponible | dc.date.available | 2011-04-04T11:47:43Z | |
Fecha de publicación | dc.date.issued | 2008-05-16 | |
Cita de ítem | dc.identifier.citation | JOURNAL OF BASIC MICROBIOLOGY, Volume: 49, Issue: 2, Pages: 135-141, 2009 | en_US |
Identificador | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119138 | |
Resumen | dc.description.abstract | The yeast Xanthophyllomyces dendrorhous is biotechnologically important due to its ability to produce the pigment astaxanthin, but is poorly understood at the genetic level. This is mainly because its preservation is difficult and many of the mutants obtained are unstable. The objectives of the present work were (i) the mutagenesis X. dendrorhous and, (ii) isolation of mutants with auxotrophic markers suitable for genetic studies of the carotenogenesis pathway and sexual cycle. Additionally, two kinds of preservation methods at the laboratory level were tested for the storage of strains. A collection of X. dendrorhous mutants affected in the production of carotenoid pigments or development of sexual structures and auxotrophic requirements were isolated by treatment with N-methyl-N′-nitro-N-nitrosoguanidine and the antibiotic nystatin. From a detailed analysis about the requirements of auxotrophic mutants the ARG7, ARG3 and PRO3 loci can be defined in this yeast. Among the methods assayed for the long-term preservation of X. dendrorhous strains, the dehydrated gelatin drop method showed the highest recovery of viable yeast after storage for 65 months. No changes in auxotrophic properties and in macro or micro morphology were observed after applying the latter method. | en_US |
Patrocinador | dc.description.sponsorship | This work was supported by the Cooperation Grant between Universidad de Chile (Chile) and C.S.I.C. (Spain), and Fondecyt 1040450. We thank Maria Teresa Rejas for her assistance in Electronic microscopy, Fundación María Ghilardi Venegas for the graduate scholarship to P. Retamales and Deutscher Akademischer Austauschdienst (DAAD) for a graduate scholarship to P. Lodato. | en_US |
Idioma | dc.language.iso | en | en_US |
Publicador | dc.publisher | WILEY-V C H VERLAG GMBH | en_US |
Palabras claves | dc.subject | Long-term storage | en_US |
Título | dc.title | Isolation, characterization and long term preservation of mutant strains of Xanthophyllomyces dendrorhous | en_US |
Tipo de documento | dc.type | Artículo de revista |
Descargar archivo
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
Artículos de revistas
Artículos de revistas