Mostrar el registro sencillo del ítem

Autordc.contributor.authorBaeza Cancino, Marcelo 
Autordc.contributor.authorRetamales Molina, Patricio es_CL
Autordc.contributor.authorSepúlveda, Dionisia es_CL
Autordc.contributor.authorLodato, Patricia es_CL
Autordc.contributor.authorJiménez, Antonio es_CL
Autordc.contributor.authorCifuentes Guzmán, Víctor es_CL
Fecha ingresodc.date.accessioned2011-04-04T11:47:43Z
Fecha disponibledc.date.available2011-04-04T11:47:43Z
Fecha de publicacióndc.date.issued2008-05-16
Cita de ítemdc.identifier.citationJOURNAL OF BASIC MICROBIOLOGY, Volume: 49, Issue: 2, Pages: 135-141, 2009en_US
Identificadordc.identifier.urihttps://repositorio.uchile.cl/handle/2250/119138
Resumendc.description.abstractThe yeast Xanthophyllomyces dendrorhous is biotechnologically important due to its ability to produce the pigment astaxanthin, but is poorly understood at the genetic level. This is mainly because its preservation is difficult and many of the mutants obtained are unstable. The objectives of the present work were (i) the mutagenesis X. dendrorhous and, (ii) isolation of mutants with auxotrophic markers suitable for genetic studies of the carotenogenesis pathway and sexual cycle. Additionally, two kinds of preservation methods at the laboratory level were tested for the storage of strains. A collection of X. dendrorhous mutants affected in the production of carotenoid pigments or development of sexual structures and auxotrophic requirements were isolated by treatment with N-methyl-N′-nitro-N-nitrosoguanidine and the antibiotic nystatin. From a detailed analysis about the requirements of auxotrophic mutants the ARG7, ARG3 and PRO3 loci can be defined in this yeast. Among the methods assayed for the long-term preservation of X. dendrorhous strains, the dehydrated gelatin drop method showed the highest recovery of viable yeast after storage for 65 months. No changes in auxotrophic properties and in macro or micro morphology were observed after applying the latter method.en_US
Patrocinadordc.description.sponsorshipThis work was supported by the Cooperation Grant between Universidad de Chile (Chile) and C.S.I.C. (Spain), and Fondecyt 1040450. We thank Maria Teresa Rejas for her assistance in Electronic microscopy, Fundación María Ghilardi Venegas for the graduate scholarship to P. Retamales and Deutscher Akademischer Austauschdienst (DAAD) for a graduate scholarship to P. Lodato.en_US
Idiomadc.language.isoenen_US
Publicadordc.publisherWILEY-V C H VERLAG GMBHen_US
Palabras clavesdc.subjectLong-term storageen_US
Títulodc.titleIsolation, characterization and long term preservation of mutant strains of Xanthophyllomyces dendrorhousen_US
Tipo de documentodc.typeArtículo de revista


Descargar archivo

Icon

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem